[899] Chromophobe Renal Cell Carcinoma – Chromosomal Aberration Variability. An Array CGH and FISH Analysis of 37 Cases

Ondrej Hes, Maris Sperga, Petr Martinek, Tomas Vanecek, Fredrik Petersson, Milan Hora, Michal Michal. Charles University, Medical Faculty and University Hospital, Plzen, Czech Republic; East University, Riga, Latvia; National University Health System Hospital, Singapore, Singapore

Background: Chromophobe renal cell carcinomas (CRCCs) are characterized by loss of genetic material most commonly involving chromosomes: 1, 2, 6, 13, 17, 21. The objective of this study was to map the spectrum of chromosomal aberrations in a larger cohort of CRCC.
Design: 546 CRCCs were reviewed and graded according to Paner grading sytem (PG). CRCCs were divided into PG 1-3, sarcomatoid, and aggressive (with confirmed metastatic disease) groups. ArrayCGH and FISH analysis were applied to 42 samples from 37 cases with well-preserved DNA.
Results: Multiple losses as well as gains were detected in different chromosomes. Only the most frequent changes (involving ≥50 % cases) are shown.
Grade 1 (8 cases): -1(6/8), -2(7/8), -5(4/8), -6(5/8), -10(6/8), -13(5/8), -17(7/8), -21(5/8).
+4(4/8), +7(4/8), +9(4/8), +12(4/8), +14(4/8), +16(4/8), +19(5/8), +20(4/8), +22(4/8).
Grade 2 (9 cases): -1(5/9), -2(6/9), -10(5/9), -17(6/9).
+7(5/9), +19(6/9).
Grade 3 (7 cases): -1(5/7), -2(4/7), -6(5/7), -10(5/7).
+3(4/7), +4(4/7), +5(5/7), +7(5/7), +8(4/7), +9(5/7), +15(4/7), +18(4/7), +19(6/7), +20(5/7).
Aggressive, grade 2 and 3 (8 cases): -1(7/8), -2(7/8), -6(7/8), -10(7/8), -13(6/8), -17(5/8), -21(5/8).
+4(5/8), +7(5/8), +12(4/8), +15(4/8), +16(4/8), +19(4/8), +20(5/8), +22(4/8).
Sarcomatoid- epithelial component (5 cases): losses and gains were detected in ≤ 50% of the cases.
-sarcomatoid component (5 cases): -10(3/5), -17(3/5).
+3(5/5), +4(5/5), +5(3/5), +7(5/5), +8(5/5), +9(4/5), +11(4/5), +12(4/5), +14(4/5), +15(5/5), +18(4/5), +19(3/5), +20(3/5), +22(3/5).
Conclusions: CRCCs showed a significantly broader spectrum of chromosomal aberrations than previously recognized. While previously published chromosomal losses were found in our cohort, gains of multiple chromosomes were also identified. The most frequently detected gains involved chromosomes 7 (24/42), 19 (24/42), 4 (23/42), 20 (21/42), 15 (20/42).
Study was supported by the Charles University Research Fund (project number P36).
Category: Genitourinary (including renal tumors)

Tuesday, March 5, 2013 9:30 AM

Poster Session III # 86, Tuesday Morning

 

Close Window