Utilizing Image Analysis To Correlate Subsets of Tumor Infiltrating Lymphocytes with Prognosis in Colon Cancer Patients
Younghoon Kim, Jeong Mo Bae, Nam-Yun Cho, Gyeong Hoon Kang. Seoul National University Hospital, Seoul, Korea; Seoul National University College of Medicine, Seoul, Korea; Cancer Research Institute, Seoul, Korea
Background: Tumor-infiltrating lymphocytes are white blood cells that have left the bloodstream and migrated into a tumor, and subset of the cells possesses capacity to kill tumor cells. Although high density of subset of TIL (e.g.: Tregs) is prognostic in various human carcinomas, its roll in colorectal carcinoma is controversial.
Design: Density of TILs bearing CD3, CD45RO, CD8, or FOXP3 was measured by Aperio Imagescope from TMA samples obtained from 767 patients diagnosed as colorectal carcinoma between 2004 and 2006.
Results: TIL subset densities showed strong correlations between each other. CD45RO+, CD8+, and FOXP3+ densities were significantly associated with improved progression free survival (PFS) and overall survival (OS) regardless of chemotherapy status. The significance was confirmed by multivariate analyses. However, FOXP3+ density showed significant correlation with PFS only in low CD8+ or CD45RO densities. CD45+ density was positively correlated with PFS in MSI-H patients, which was confirmed by univariate and multivariate analyses.
Conclusions: CD8, CD45RO, and FOXP3 were meaningful prognostic factors even for patients with adjuvant therapy. Among the TIL subsets, CD45RO had prognostic value over MSI-H patients. Therefore, subset of TILs is considered a potential marker for colorectal carcinoma patients in various conditions.
Wednesday, March 6, 2013 9:30 AM
Poster Session V # 95, Wednesday Morning