[652] The Incidence of EBV and Her2 Positivity in Gastric Cancers

Cigdem Irkkan, Serdar Balci, Gaye Guler Tezel, Bulent Akinci, Bulent Yalcin, Gulnur Guler. Yildirim Beyazit University, Ankara, Turkey; Hacettepe University, Ankara, Turkey

Background: After realisation of H.pylori pathogenesis, gastric carcinoma incidence declined in developed countries.New associations like Ebstein Barr Virus (EBV) infection and Targeted therapy related comprehensive investigations of prevelance, prognostic value and associations of Her2 positivity emerged recently. Here we searched the association between EBV positivity and Her2 status of gastric carcinoma.
Design: From 106 gastrectomy specimens, most intestinal differentiated tumor tissue blocks were selected. Her2 was searched with clone:4B5 immunohistochemistry (IHC) and Brightfield Dual Silver In situ hybridisation (ISH) methods and EBV status is searched with Inform EBER ISH method; all techniques performed on the same automated platform.
Results: Her2 positive, EBER positive and tumors negative for both were different in terms of T stage and median survival but not other features.

Histomorphologic and survival features according to Her2 and EBV status
 All cases (n=106*)Her2 positive (n=13)EBER positive (n=7)Negative for both (n=85)p
Age59.5±13.564.6±12.662.0±16.658.7±13.40.307
Gender M:F79:2711:27:060:250.153
Histologic subtypeDiffuse22(20.8%)1(7.7%)1(14.3%)20(23.5%)0.073
 Intestinal49(46.2%)10(76.9%)1(14.3%)38(44.7%) 
 Mixed25(23.6%)2 (15.4%)3 (42.9%)20 (23.5%) 
 Undiff10(9.4%)02(28.6%)7(8.2%) 
TT17(6.6%)1(7.7%)06(7.1%)0.038
 T212(11.3%)1(7.7%)011(12.9%) 
 T323(21.7%)7(53.8%)016(18.8%) 
 T464(60.4%)4(30.8%)7(100%)52(61.2%) 
Lymph nodeNegative22(21.4%)3(25.0%)018(21.4%)0.420
 Positive81(78.6%)9(75.0%)6(100%)66(78.6%) 
MetastasisM046(59.7%)5(50.0%)4(66.7%)36(60.0%)0.777
 M131(40.3%)5(50.0%)2(33.3%)24(40.0%) 
Tumor size(cm)5.5±3.15.1±2.45.6±3.25.6±3.20.870
LVI61(57.5%)4(30.8%)5(71.4%)52(61.2%)0.089
PNI42(39.6%)4(30.8%)4(57.1%)33(38.8%)0.512
Median survival(months)182011190.005
*One case was positive for EBER and Her2 IHC and was borderline in ISH. It is included under descriptive statistics but discarded from statistical comparisons.Percentages are given for columns.


Conclusions: EBV positive group had significantly shorter overall survival in univariate but not in multivariate analyses. This relation needs to be confirmed in larger cohorts. Ratios of EBV positivity (7.8%) and Her2 amplification (11%) of gastric carcinomas in our country are lower than western countries. Lower Her2 rates is thought to be related with higher diffuse type carcinoma incidence in our country.
Category: Gastrointestinal

Wednesday, March 6, 2013 1:00 PM

Poster Session VI # 100, Wednesday Afternoon

 

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