Rabbit Polyclonal Anti-SOX10 Is a Reliable IHC Marker for Melanoma and Its Mimics
George G Yang, Ashot Minasyan, Jeff Gordon, Mike Lacey, Kurt Lundquist. Cell Marque Incorporation, Rocklin, CA
Background: SOX10 is a transcription factor that participates in melanocytic and Schwann cell differentiation. It has been reported that SOX10 antibody is a sensitive marker for melanomas. However, thus far the primary antibody against SOX10 protein in the research and publications has been antiserum from goat, which hinders the antibody's acceptance, particularly in laboratories for clinical diagnosis. Here we report the assessment of SOX10 expression of melanomas, carcinomas, mesenchymal neoplasms, nerve tumors, and mesotheliomas using rabbit polyclonal anti-SOX10 antibody.
Design: In this study, we evaluated this rabbit polyclonal anti-SOX10 for benign tissue cross-reactivity, neoplastic expression, sensitivity and specificity on whole tissue sections and tissue microarray by routine immunohistochemistry.
Results: The results are summarized in the table 1.
|Neoplasm||Positive cases/Total cases||Sensitivity (%)|
|Melanoma, spindle cell (metastasis to lymph node||3/3||100%|
|Malignant peripheral nerve sheath tumor||3/4||75%|
|Granular cell tumor||5/5||100%|