Comparison of Stromal Expression of CD34 and Neoplastic Cell Expression of HMB-45 in Primary Melanomas and Melanocytic Nevi of Skin
Shahram Sabeti, Mohammad Khabazian, Mihan Pourabdollah Toutkaboni, Farhad Malekzad. Shahid Beheshti University of Medical Sciences, Loghman Hakim Hospital, Tehran, Islamic Republic of Iran; Shahid Beheshti University of Medical Sciences, NRITLD, Tehran, Islamic Republic of Iran; Shahid Beheshti University of Medical Sciences,Dermatology Research Center, Shohadaie Tajrish Hospital, Tehran, Islamic Republic of Iran
Background: Melanoma is a clinically and histopathologically challenging neoplasm;some of its variants being capable of mimicking banal melanocytic nevi. Immunohistochemistry,due to its usefulness, handiness and low cost, has been the most useful adjunct to light microscopy for distinguishing this neoplasm from its benign counterparts and other mimickers. Stromal remodeling leading to loss of CD34(+) fibrocytes has been extensively studied in many neoplasms and can be a useful adjunct in differentiating benign from malignant neoplasms. On the other hand,one of the well-known,highly specific melanoma markers is HMB-45.
Design: We investigated the rate of immunohistochemical stromal expression of CD34 and compared it with the neoplastic cell expression of HMB-45 in malignant melanomas and melanocytic nevi of skin.
Results: We examined 22 cases of invasive melanomas and 20 cases of banal melanocytic nevi of skin. the sensitivity and specificity obtained for CD34 as a discriminant marker were 90.9% and 90.5% and those obtained for HMB-45 were 91% and 90%,respectively.