[476] Evaluation of Fumarate Hydratase and Hypoxia-Associated Proteins in HLRCC Syndrome and Sporadic Cutaneous Leiomyomas

Jyoti P Kapil, Nitin Chakravarti, Jonathan L Curry, Wei-Lien Wang, Victor G Prieto, Michael T Tetzlaff, Carlos Torres-Cabala. MD Anderson Cancer Center, Houston, TX

Background: Hereditary leiomyomatosis and renal cell carcinoma(HLRCC) is a rare autosomal dominant syndrome with predispositions for cutaneous and uterine leiomyomas and aggressive RCC. It is caused by a germ-line inactivating mutation in fumarate hydratase(FH) gene, thought to initiate a pseudohypoxic drive culminating in stabilization of hypoxia inducible factor-1a(HIF-1a). Little is known about the expression of the proteins involved in the postulated altered molecular pathway in HLRCC-related and sporadic cutaneous leiomyomas.
Design: 12 cutaneous leiomyomas and 1 smooth muscle tumor of uncertain malignant potential(SMTUMP) were examined for IHC expression of FH, HIF-1α, HIF-2α, and HIF-1α-associated downstream proteins lactate dehydrogenase-A(LDH-A) and glucose transporter-1(GLUT-1). Each marker was evaluated for intensity of expression(scale of 0 to 3), percentage of positive cells(0,1:<5%, 2:5-50%, 3:greater than 50%), and subcellular localization(nuclear, cytoplasmic, or both).
Results: The clinical and histologic features of the 13 cases:6 HLRCC pilar leiomyomas, 4 sporadic pilar leiomyomas, 2 angioleiomyomas and 1 SMTUMP were selected from patients(range 29-89 y/o) with a M:F ratio 4:5. Sites: arm(4), leg(4), shoulder(2), trunk(2), and foot(1). HLRCC patients also presented with metastatic renal carcinoma(1) and uterine leiomyomas(1).

Expression of FH and Hypoxia Markers in HLRCC and Sporadic Cutaneous Leiomyomas
Lesions(#Tumors/#Patients)FH*HIF-1α*HIF-2α*LDH-A*GLUT-1*
HLRCC leiomyomas(6/2)0,03,33,32.5,30,0
Sporadic leiomyomas(4/4)1,12,2.53,31.5,1.50,0.5
Sporadic angioleiomyomas(2/2)2.5,2.52.5,33,31.5,20,0
STUMP(1/1)2,33,33,32,30,0
*All values given as average for intensity and percentage of positive cells(i,%)


Conclusions: In comparison to the sporadic counterparts, HLRCC leiomyomas lack expression of FH. This finding may be very useful in clinical practice. Low FH expression in sporadic pilar leiomyomas may indicate a role of FH in these tumors; angioleiomyomas seem to harbor FH-independent mechanisms. Higher expression of LDH-A in HLRCC compared to other tumors supports an intrinsic activation of HIF-1a. Overexpression of HIF-1a and HIF-2a was detected in all the analyzed lesions, suggesting a common final hypoxic/pseudohypoxic activation in both HLRCC and sporadic smooth muscle lesions. Lack of expression of GLUT-1 in all the leiomyomas—in contrast to that reported in HLRCC renal carcinomas, is intriguing and may suggest a different metabolic final pathway in these lesions.
Category: Dermatopathology

Wednesday, March 6, 2013 9:30 AM

Poster Session V # 70, Wednesday Morning

 

Close Window