Evidence That Judicious Use of a Cellient™ Cell Block and p16/Ki67 Immunohistochemistry (IHC) in Pap Tests Improves the Prediction of Biopsy Findings of CIN2 or Worse
Wei Xing, Andrew Fischer, April Y Hou, Zhong Jiang. University of Massachusetts Medical School, Worcester, MA; Brandeis University, Waltham, MA
Background: P16 and Ki67 stains are increasingly used in cervical biopsies. Little data is available for use of p16 and Ki67 stains in cell blocks of Pap tests.
Design: 56 Pap tests between January 2010 and March 2012 with equivocal diagnoses on Pap smear were examined with H&E stained Cellient™ cell blocks and p16 and/or Ki67 immunohistochemistry (IHC). Cellient utilizes a flow-through embedding technique and automatically positions cells at a defined plane in paraffin to maximize cell yield. The final pathologist diagnoses based on review of the cell block and IHC findings were compared to the cytotechnologist diagnoses based on the Pap test alone. The diagnoses based on the Pap test alone and IHC slides were confirmed in a subset of cases. Two-tailed chi square test was used for statistical analysis.
Results: The cytotechnologist Pap test diagnoses on the 56 cases are shown in Table 1.
|Cytotechnologist||Pathologist diagnoses integrating cell block with p16/Ki67 IHC|
|Diagnoses on follow up|