Comparison of Single vs. Concurrent Multiple HPV Genotypes Infection Pattern in Cervical Cytology of Women with H-SIL
Francisco Beca, Elisabete Rios, Patricia Pontes, Isabel Amendoeira. IPATIMUP – Institute of Molecular Pathology and Immunology of the University of Porto, Porto, Portugal; Faculty of Medicine of the University of Porto, Porto, Portugal; Centro Hospital de São João, EPE, Porto, Portugal
Background: The contribution of human papillomavirus (HPV) types to the carcinogenesis of cervical cancer has been established for a long time. However, the role of phylogenetically related HPV genotypes and rare variants remains uncertain, as well as the influence of concurrent multiple HPV genotypes infection. The present study aims at studying the pattern of prevalence of several HPV genotypes infecting women with single vs. concurrent multiple HPV genotypes infection with a H-SIL diagnosis in a cervical cytology.
Design: It was conducted a cross-sectional study using Thin-Prep® liquid-based cervical cytology specimens with the diagnosis of H-SIL, in which HPV genotype was sequentially tested. Women with the diagnosis of H-SIL and vaginal specimens or unavailable genotyping results were excluded. Genotypes were determined with a PapilloCheck® system (Greiner Bio-One, Frickenhausen, Germany), a DNA-Chip for the type-specific identification of 18 high-risk and 6 low-risk types of HPV.
Results: Between 2008 and 2011, a total of 1394 liquid-based cervical cytology samples were analysed and the diagnosis of H-SIL made in 207 (14.85%; 95%CI: 13.08-16.82) specimens. Of these, 176 (85.02%; 95%CI: 0.79-89.29) had available and positive HPV genotyping result. Mean age of these women was 39.35 years old (95%CI: 37.81-40.85), being HPV16 the most prevalent genotype (48.30%; 95%CI: 41.03-55.64) followed by HPV31 (14.20%; 95%CI: 9.75-20.18). HPV18 was detected in 5.11% (95%CI: 2.58-9.56) of the samples. Concurrent multiple HPV genotypes were detected in 36.72% (95%CI: 29.55-43.89) of the samples. The prevalence of the nine most common HPV genotypes detected varied significantly according to the presence of single vs. concurrent multiple HPV genotypes (p=0.016). Moreover women with concurrent multiple HPV genotypes were on average 3.53 (95%CI: 0.43-6.64) years younger than women with single genotype infection.
Conclusions: Our results suggest that women with multiple vs. single genotype HPV infection differ in terms of age and distribution of the most prevalent HPV genotypes. Moreover, we provide further evidence of the predominance of HPV16 in H-SIL lesions of the uterine cervix.
Monday, March 4, 2013 9:30 AM
Poster Session I Stowell-Orbison/Surgical Pathology/Autopsy Awards Poster Session # 48, Monday Morning