Vesicular-Lamellated Bodies in a Case of Chromophobe Renal Cell Carcinoma: A Transitive Form between Mitochondria and Vesicle?
Amanda Krausert, Cheddhi Thomas, Rosemary Wieczorek, Nicholas Cassai. NYU School of Medicine, New York, NY; New York Harbor Healthcare System (NY VA), New York, NY
Background: Chromophobe renal cell carcinoma (CRCC) has classically been characterized as being Hale's colloidal iron positive and ultrastructurally containing abundant cytoplasmic microvesicles and mitochondria with tubulo-vesicular cristae and dense plaques. Here we present a case of CRCC with unusual electron microscopic findings.
Design: Tissue was fixed in 3% phosphate buffered glutaraldehyde, post fixed in 1% osmium tetroxide and embedded in Epox A12. Ultrathin sections were cut at 70nm and stained with uranyl acetate and lead citrate. Electron microscopic examination was performed on a JEM 1010 microscope.
Results: Ultrastructural examination revealed numerous vesicular bodies along with abnormal vesicular mitochondria containing dense plaques. In addition, numerous unusual bodies with a lamellated structure were observed, some of which contained vesicles.
Conclusions: These unusual structures, which we call vesicular-laminated bodies, have not been previously observed in CRCC. Given that these lamellated structures have foci of vesicle inclusion, these bodies may represent a transitive form between mitochondria and the cytoplasmic vesicles seen in CRCC.
Wednesday, March 6, 2013 9:30 AM
Poster Session V # 303, Wednesday Morning