CD123 Is a Useful Immunohistochemical Marker for Colonic Graft Versus Host Disease
Jingmei Lin, Shaoxiong Chen, Zijin Zhao, Oscar W Cummings, Rong Fan. Indiana University School of Medicine, Indianapolis, IN
Background: Hematopoietic stem cell transplantation provides a potential cure for many immunohematopoietic disorders. However, the efficacy of this treatment is greatly hampered by graft versus host disease (GVHD) and opportunistic infection. The gastrointestinal tract is one of the main target organs for GVHD and infections, particularly in form of cytomegalovirus colitis. Cytomegalovirus infections can coexist or superimpose GVHD, sometimes during anti-GVHD treatment. The presence of crypt apoptosis is one of the major criteria when diagnosing GVHD; however, it is also a common finding in cytomegalovirus colitis. Thus, the definitive histopathological diagnosis of gastrointestinal GVHD is often challenging or impossible without reliable ancillary markers. Some previous studies suggested a role of CD123 and C4d in intestinal GVHD pathophysiology.
Design: In this study, we retrospectively reviewed 38 colonic biopsies from 34 patients who received stem cell transplantation and had a diagnosis of GVHD (20 cases of grade 1-2 and 18 cases of grade 3-4) and 14 colon biopsies from 14 patients who had cytomegalovirus colitis without history of stem cell transplantation, and studied the expression of CD123 and C4d in both groups.
Results: Our data demonstrated significantly increased expression of CD123 in the GVHD group (total 65.8%; 60% in grade 1-2; 72.2% in grade 3-4) compared to the cytomegalovirus group(14.3%; p<0.01); there was no significant difference of C4d expression between the two groups (68.4% and 42.9% respectively; P>0.05).
Conclusions: Our study indicates that for patients who are highly suspicious for gastrointestinal GVHD in the presence of confounding factors, CD123 might be a useful ancillary marker.
Category: Quality Assurance
Tuesday, March 5, 2013 9:30 AM
Poster Session III # 296, Tuesday Morning