Flow Cytometric Characterization of Peripheral Blood Fibrocytes in Lung Transplant Patients
Adeel M Raza, Yuli Zu, Philip T Cagle, Roberto Barrios, Hidehiro Takei, Yimin Ge, Audrey Ponce De Leon, April Ewton. Methodist Hospital, Houston, TX
Background: Fibrocytes are bone marrow derived mesenchymal cells with properties of both macrophages and fibroblasts that participate in tissue repair and fibrosis. Increased circulating fibrocytes can be present in patients with pulmonary parenchymal diseases, nephrogenic fibrosis, pulmonary hypertension and in autoimmune disorders. The antigenic characterization of circulating fibrocytes in lung transplant patients may yield clues to disease progression, but have not been extensively studied.
Design: Pheripheral blood from 15 lung transplant patients (n=15) and 20 healthy controls (n=20) were examined by flow cytometry to quantify circulating fibrocytes among lung transplant patients. The panel of antibodies includes CD14, CD45, CD34 and collagen.
Results: The percentages of peripheral blood fibrocytes are significantly higher in lung transplant patients (9/15, 60%) (p=0.00027)
compared to control patients.
These fibrocytes are characterized by expression of CD34, CD14, CD45 and collagen.
Conclusions: Increased circulating fibrocytes are seen in majority of lung transplant patients. Our study showed significantly higher percentage of circulating fibrocytes in lung transplant patients compared to patients without transplant. Circulating fibrocytes could serve as a clinical biomarker and can provide a method of monitoring fibrosis of the transplanted lungs and may provide a therapeutic target for chronic graft rejection. However further studies are needed to determine the correlation between risk of fibrosis after lung transplantation and percentage of circulating fibrocytes.
Tuesday, March 5, 2013 1:00 PM
Poster Session IV # 300, Tuesday Afternoon