Overexpression of the Tumor Stomal Marker Periostin Is Correlated with p53 Levels in Non-Small Cell Lung Cancer (NSCLC) Detected by Immunohistochemistry Using Lung Cancer Tissue Microarray (TMAs)
Thomas J Gniadek, Caitlin Choi, Yuan Tian, Hui Zhang, Edward Gabrielson, Frederic Askin, Qing K Li. Johns Hopkins University School of Medicine, Baltimore, MD
Background: Periostin is a recently identified stromal marker. Its overexpression has been related to aggressive tumor behavior, advanced tumor stage, and increased metastatic potential in several types of cancer, including breast and prostate cancer. Recent studies have also suggested that periostin may be involved in NSCLC and that elevated levels of p53 and cyclin D1 may play a role in periostin expression. In this study, we evaluate the expression of periostin in different subtypes of NSCLC and correlate its expression with p53 and cyclinD1 levels.
Design: Using pathology archives from our academic center, two lung cancer tissue microarrays (TMAs) were constructed using surgical material, which include 81 cases of adenocarcinoma (ADC) and tumor-matched normal lung tissues as well as 91 cases of squamous cell carcinoma (SQCC). The anti-human periostin mab was used at 1:1000 dilution. Anti-p53 and cyclinD1 were used per Johns Hopkins histology standard protocols. The staining patterns were scored semi-quantitatively as: 0 negative, 1 weak and focal, and 2 strong and diffuse. The association between Periostin and p53/cyclinD1 expression was analyzed using Goodman and Kruskai's gamma methods.
Results: The overall expression of periostin was high in 52% of ADC and 51% of SQCC. The expression of p53 was detected in both ADC and SQCC. There was a statistically significant association between periostin overexpression and elevated p53 levels in SQCC, but not in ADC (p-value: 0.0026 vs 0.13, respectively). The expression of cyclinD1 was not associated with periostin expression in either SQCC or ADC TMAs (p-values: 0.59 and 0.67).
Conclusions: Within this data set, the correlation between overexpression of periostin by tumor stromal fibroblasts and elevated p53 levels differed between SQCC and ADC of the lung. Our data suggest that overexpression of periostin may involved in p53 signaling pathway in certain subtype of cancer. While research continues into the mechanism and prognostic significance of periostin expression, these data may help refine periostin as a prognostic marker and lead to a better understanding of the tumor biology that leads to increased expression.
Wednesday, March 6, 2013 1:00 PM
Poster Session VI # 298, Wednesday Afternoon