[1667] Different Collagen Types Show Variable Increases from Early to Late Stages of Hepatitis-C Related Liver Fibrosis

Wei Chen, Jonathan B Rock, Martha M Yearsley, Linda D Ferrell, Wendy L Frankel. Ohio State University Wexner Medical Center, Columbus, OH; University of California, San Francisco, CA

Background: During progression from normal liver to cirrhosis, significant changes occur in extracellular matrix (ECM). Total collagen (Co) increases 3 to 10 fold with an abnormal increase in fibril-forming Co and other ECM molecules. Little is known regarding changes each Co type undergoes during fibrogenesis. We evaluated 4 common types of Co and vitronectin at different stages of hepatitis-C related liver fibrosis to assess differential staining.
Design: 73 liver core biopsies from Hepatitic C patients (2007-2012) were identified. Transplanted livers and cases with other known liver diseases, except steatosis, were excluded. Cores were immunostained for Co III, IV, VI, Vitronectin, and Trichrome (Co I). Staining intensity was graded as negative (0), weak (1), and strong (2); and distribution as patchy if <50% of the portal areas stained (1), or diffuse (2). The final score was calculated as the product of intensity and distribution. Statistical analysis was performed using an unpaired student's T-test.
Results: Patient age ranged from 48 to 55, with male predominance in all stages. Co III, IV, VI, vitronectin and trichrome all showed increases from early to late stages of fibrosis (P <0.0001), but with temporal and quantitative differences. Co IV and Trichrome show greatest differences from early to late fibrosis, while Co VI shows strong reactivity even in stage 1.

ECM Scores
Stage# of CasesCo IIICo IVCo VIVitronectinTrichrome (Co I)
1211.7 ± 0.60.6 ± 0.73.0 ± 1.21.4 ± 0.62.0 ± 0.6
2202.0 ± 1.00.7 ± 0.84.6 ± 1.31.5 ± 1.02.5 ± 0.9
3172.5 ± 1.52.9 ± 1.35.9 ± 0.53.2 ± 1.55.2 ± 1.1
4154.8 ± 1.54.7 ± 1.46.0 ± 0.03.7 ± 2.05.4 ± 1.0
Average (1 + 2)411.8 ± 1.10.6 ± 0.73.8 ± 1.51.4 ± 0.82.2 ± 0.8
Average ( 3 + 4)323.6 ± 1.93.7 ± 1.65.9 ± 0.43.4 ± 1.75.3 ± 1.0





Conclusions: Co IV and Trichrome appear to be the most useful discriminators between early and late stages of fibrosis. Co VI shows strong expression in early fibrosis while Co IV is weaker than all others until stage 3 fibrosis. Further studies will be helpful to evaluate usefulness in staging and reversibility of fibrosis.
Category: Liver

Wednesday, March 6, 2013 1:00 PM

Poster Session VI # 111, Wednesday Afternoon

 

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