Curcumin Enhances Mesangial Repair by Stem Cells in Light Chain-Mediated Glomerular Injury
Jiamin Teng, Elba A Turbat-herrera, Guillermo A Herrera. Louisiana State University Health Science Center, Shreveport, LA
Background: Light chain deposition disease (LCDD)-associated glomerulopathic light chains (GLCs) induce glomerular injury characterized by increased mesangial extracellular matrix (ECM) and, in the early stages, mesangial cellular proliferation. Repair of the damaged mesangium by stem cells is complicated by the need to dispose of the increased ECM present. Curcumin has been proven to enhance apoptosis in this setting. The present study was designed to evaluate the effect of curcumin on the altered and expanded ECM.
Design: Human mesangial cells (HMCs) were incubated with LCDD-GLCs purified from the urine of patients with kidney biopsy proven LCDD. HMCs were seeded in glass bottom plates and incubated in the 6D living cell platform until about 40% confluent. HMCs were made quiescent and then incubated with the G-LCs (10 ug/ml) for 4 days. Then these cells were added curcumin (10uM) for 4 days. Annexin-V FITC and propidium iodine were used to evaluate apoptosis. The entire process was monitored using the 6D imaging system. Controls included GLCs-treated HMCs without incubation with curcumin and camptothecin (10μM) to enhance apoptotic events as a positive control.
Results: LCDD-LCs induced apoptosis in mesangial cells and participated in the ECM production, as previously documented. Curcumin enhanced the apoptosis of mesangial cells and clearing of the ECM that LCDD-LCs generated. This resulted in effective seeding of the mesangium by the stem cells and eventual production of new, biochemically normal ECM by the stem cells leading to mesangial healing.
Conclusions: Curcumin enhances mesangial cell apoptosis and contributes to the repair of the damaged glomerular by the stem cells by facilitating removal of the abnormal matrix present. Stem cells are unable by themselves to effectively clear the expanded ECM. Therefore, the role of curcumin is important in mesangial repair.
Category: Kidney (does not include tumors)
Tuesday, March 5, 2013 11:00 AM
Proffered Papers: Section H, Tuesday Morning