[1629] Expression of CD71 in Mesangial Cells of IgA Nephropathy and Proliferative Parietal Epithelial Cells of Immune Complex Crescentic Glomerulonephritis

Wei Li, Ping Zhang. William Beaumont Hospital, Royal Oak, MI

Background: The transferrin receptor (CD71) is an integral membrane protein that mediates the uptake of transferrin-iron complexes. CD71 has been shown to be abnormally expressed in a variety of immune-related diseases. The mechanisms of CD71 involving in the process of immune-related diseases are unclear. This study aims to evaluate immunohistochemical expression of the CD71 in IgA nephropathy and crescentic glomerulonephritis and assess its value in the diagnosis of some glomerulonephritis.
Design: The renal biopsy tissue sections of 28 IgA nephropathy (IGAN), 16 non-IgA immune complex crescentic glomerulonephritis (ICCGN), 13 Pauci-immune crescentic glomerulonephritis (PICGN), 19 acute kidney injury (AKI) were immunostained for CD71. The number of immunoreactive glomerular mesangial, endothelia and epithelial cells was scored as 0-3. Staining intensity was graded as 0 -3+ The total immunoreactivity score (IRS) of immunostaining was calculated by adding the percentage score (0 to 3) of immunoreactive cells to the intensity score (0 to 3).
Results: The number of CD71 positive mesangial cells and the intensity of immunostaining were significantly increased in IGAN compared with ICCGN, AKI and PICGN (P < 0.01). The differences was quantified by calculating the IRS (Table 1).

Table 1: Total Immunorectivity Score No. (%) of CD71 in Mesangial Cells.
Score023456 
       Total Case No.
IGAN01(4)2(7)5(18)14(50)6(21)28
AKI4(21)13(69)1(5)1(5)0019
ICCGN4(25)10(62)2(13)00016
PICGN3(24)8(62)1(7)1(7)0013


The number of CD71 positive proliferative parietal epithelial cells and the intensity of immunostaining were significantly increased in ICCGN compared with PICGN (P < 0.001). The differences was quantified by calculating the IRS (Table 2).

Table 2: Total Immunorectivity Score No.(%) of CD71 in Parietal Epithelial Cells.
Score023456 
       Total case No.
ICCGN002(12)2(12)8(51)4(25)16
PICGN4(31)7(53)1(8)1(8)0013


No increased expression of CD71 was observed in glomerular endothelial cells in all study groups.
Conclusions: Increased expression of CD71 in mesangial cells IGAN supports the conclusion of previous study that CD71 serves as an IgA receptor mediating mesangial deposition of IgA complexes in IGAN. An enhanced expression of CD71 in proliferative parietal epithelial cells in ICCGN suggests that CD71 could also play an important role in triggering the immune activation involving crescent formation. CD71 staining may serve as a useful marker in aiding diagnosis of IGAN and ICCGN.
Category: Kidney (does not include tumors)

Monday, March 4, 2013 1:00 PM

Poster Session II # 236, Monday Afternoon

 

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