The Subcapsular Blue Strip Width: A New Marker for Evaluating the Residual Potential Nephrogenesis in the Newborn Kidney
Gavino Faa, Daniela Fanni, Clara Gerosa, Matteo Fraschini, Sonia Nemolato, Giovanni Ottonello, Irene Messana, Tiziana Cabras, Guido Monga, Peter Van Eyken, Massimo Castagnola, Vassilios Fanos. University of Cagliari, Cagliari, Italy; University of Novara, Novara, Italy; K.U. Leuven, Leuven, Belgium; Catholic University of Rome, Roma, Italy
Background: The multipotent cap mesenchymal cells located in a blue-appearing strip in H&E-stained sections and located under the renal capsule represent the nephron progenitor population of the newborn kidney.
Design: 30 kidneys from 15 consecutive preterm newborns of 19-38 weeks of gestation at birth, were routinely processed. Histological sections were stained with H&E. In each kidney, the mean width of the mesenchymal pluripotential cells located under the renal capsule, appearing as a “blue strip” was measured by a semi automatic segmentation method based on combination of color and texture information. A comparison with human based measurement was also performed as well as the radial glomerular count, representative of the entity of the previous nephrogenesis.
Results: A marked interindividual variability was observed in the amount of pluripotential/stem cells detected in the preterm kidneys. The width of the “blue strip” did not correlate with the gestational age of preterms at birth. Significant differences regarding the residual nephrogenic potential was observed among preterms of the same gestational age. No correlation was found between the blue strip width and the values obtained with the radial glomerular count. Finally, the semi automatic method performed as well as the human measurement but it allowed avoiding problems related to interindividual evaluation.
Conclusions: Measurement of the amount of pluripotential renal cells appears a simple and effective new tool for the evaluation of the residual potential nephrogenesis in newborn kidneys. The blue strip width should be considered complementary to previous methods, including the radial glomerular count, that give a reliable information on the previous nephrogenesis. The absence of any correlation between the blue strip width and the glomerular count (future and past of nephrogenesis) allows to hypothesize that nephrogensis should not be considered as a progressive linear process at constant speed, but as a process that could start very fast in the first period of gestation and progress at a lower speed in the final period, or vice versa.
Category: Kidney (does not include tumors)
Monday, March 4, 2013 1:00 PM
Poster Session II # 251, Monday Afternoon