Morphometric Analysis in the Diagnosis of Ductal (DCIS) and Lobular Carcinoma In Situ (LCIS)
Carlos Parra-Herran, Tyna Hope, Elzbieta Slodkowska, Fang-I Lu, Taha Rashed, Marko Katic, Guangming Han, Gina Clarke, Martin Yaffe, Sharon Nofech-Mozes. Sunnybrook Health Sciences Centre, University of Toronto, Toronto, ON, Canada; Centre for Imaging Technology Commercialization, Toronto, ON, Canada
Background: Distinction between DCIS and LCIS, while critical for margin assessment and further management (re-excision, radiation), is sometimes challenging. Immunohistochemistry for E-Cadherin and Catenin P120 has proven utility. However up to 15% of LCIS cases show E-Cadherin expression. We aim to identify quantifiable histologic features by digital image analysis that can differentiate between DCIS and LCIS as an adjunct diagnostic tool.
Design: Scanned images of 13 cases of DCIS and 14 cases of LCIS were analyzed. Three regions of solid intraductal proliferation were selected from each case and analyzed using Mercator Version 1.0 (ExploraNova, La Rochelle, France). The system detects structures based on optical density and analyzes them as closed objects.
We analyzed multiple morphometric variables.
|Nuclear density (ND)||NC/TSA|
|% Nuclear surface area||SNA/TSA*100|
|Mean nuclear area (MNA)||SNA/NC|
|Mean nuclear form factor (MNFF)||SNF/NC|
|% direct cytoplasm area (measured by the system)||SCA/TSA*100|
|% indirect cytoplasm area (calculated)||(TSA-SNA-SWS)/TSA*100)|