Analysis of Aberrant Antigenic Expression in Classical Hodgkin Lymphomas and Their Potential Role as Prognostic Factors
TuDung T Nguyen, John L Frater, Jonathan Klein, Ling Chen, Kelly V Foyil, Nancy L Bartlett, Friederike H Kreisel. Washington University, St. Louis, MO; Molecular Pathology Laboratory Network, Inc., Maryville, TN
Background: Classical Hodgkin lymphomas (cHLs) are common lymphomas with varied clinical outcomes. Previous studies have tried to predict the outcomes of cHLs using aberrant antigenic expression or other biological markers. We undertook a comprehensive immunohistochemical evaluation of 12 antigens' expression in cHLs, and correlated these findings with overall survival (OS).
Design: Immunohistochemistry for CD30, PAX5, CD3, CD2, CD4, CD5, CD7, TIA-1, MUM1, CD25, PD1, and ZAP70 was performed on diagnostic tissue of 88 patients with cHL on tissue microarrays. Immunoprofiles could be obtained for 74 cases which had sufficient tissue or tumor cells. All patients were treated with either ABVD or an ABVD-like regimen with or without radiotherapy. Percentage of positively stained Reed-Sternberg cells (RSC) and expression in tumor infiltrating T lymphocytes (TILs) were analyzed. Clinical outcome (OS) was available for 81 cases, and correlated with expression results.
Results: All cases showed expression of CD30 and MUM1, and lacked CD7. Cox proportional hazards model was used and no significant correlation was found for CD2, CD3, CD4, CD5, PAX5, TIA-1, and ZAP70 expression in RSC and OS in univariate survival analysis. PD1 and TIA-1 expression in the RSC and in the TILs also showed no significant correlation with clinical outcome. Expression of T cell antigens (CD2, CD3, CD4, CD5) and TIA-1 on RSC was associated with nodular sclerosing subtype using Chi-square test (p=0.0128). Although CD25 expression on RSC showed a trend toward clinical significance (p=0.0593) in univariate survival analysis, this was not seen in multivariate survival analysis (p=0.1603). Only the IPS (International prognostic score) remained a statistically significant predictor of OS (p=0.0022).
Conclusions: Expression of T cell antigens, PAX5, MUM1, TIA-1, ZAP70, and PD1 can be seen in cHLs, but their expression patterns do not affect the prognosis of cHLs. Although CD25 showed a trend toward clinical significance in univariate analysis, this was not shown in multivariate analysis. The IPS remains a reliable clinical predictor of OS. Additional larger studies may be needed to confirm the useful of these markers as prognostic factors in cHLs.
Monday, March 4, 2013 1:00 PM
Poster Session II # 212, Monday Afternoon