CD103 Immunoreactivity in Paraffin Sections of a Broad Spectrum of T-Cell Lymphomas
Elizabeth A Morgan, Christopher Carey, Aliakbar Shahsafaei, David M Dorfman, Scott J Rodig, Geraldine S Pinkus. Brigham and Women's Hospital, Harvard Medical School, Boston, MA
Background: CD103 is expressed by a subset of T-cell lymphomas, most notably enteropathy-associated T-cell lymphoma (EATL). Currently, flow cytometry and frozen section immunohistochemistry (IHC) represent the only available detection methods.
Design: CD103 expression in 103 paraffin sections was determined using a monoclonal antibody and an immunoperoxidase technique in the following: adult T-cell leukemia/lymphoma (ATLL) (n=6), ALK-positive anaplastic large cell lymphoma (ALCL) (7), ALK-negative ALCL (6), angioimmunoblastic T-cell lymphoma (14), primary cutaneous gamma/delta T-cell lymphoma (1), EATL (2), extranodal NK/T-cell lymphoma, nasal type (10), hepatosplenic T-cell lymphoma (2), T-cell large granular lymphocytic leukemia (1), cutaneous T-cell lymphoma/mycosis fungoides (CTCL) (6), peripheral T-cell lymphoma not otherwise specified (PTCL NOS) (37), subcutaneous panniculitis-like T-cell lymphoma (SPTCL) (4), T lymphoblastic leukemia/lymphoma (T-ALL) (5), and T-cell prolymphocytic leukemia (2).
Results: CD103 expression was strong and diffuse in 2/2 EATL and 1/37 PTCL NOS (arising in the intestine of a patient without celiac disease). In EATL, the adjacent intraepithelial lymphocytes were CD103-positive (Figure 1). A small subset of CD103-positive cells was detected in 3/4 SPTCL, 3/5 ATLL, 3/6 CTCL, 1/5 T-ALL, and 1/7 ALK-negative ALCL. The remainder were negative. Reactivity for CD103 was predominantly membranous (Figure 2).
Conclusions: This is the first successful use of a monoclonal antibody for IHC detection of CD103 in paraffin-embedded T-cell neoplasms. T-cell lymphomas of intestinal origin including EATL demonstrate strong, diffuse reactivity, confirming prior reported frozen tissue IHC results. A small subset of cells in some ATLL, CTCL and SPTCL are also reactive. The majority of other T-cell neoplasms are negative. Use of this antibody on paraffin-embedded sections is a valuable addition to the IHC evaluation of T-cell neoplasms.
Monday, March 4, 2013 1:00 PM
Poster Session II # 214, Monday Afternoon