Human Papillomavirus Detection and Genotyping in Squamous Cell Carcinomas of the Tongue
Eduardo Alcaraz-Mateos, Jose A Garcia-de Marcos, Beatriz Perez-Zafrilla, Raul Calero-Oliver, Susana Arroyo-Rodriguez, Enrique Poblet-Martinez. Hospital Universitario Morales Meseguer, Murcia, Spain; Hospital General Universitario de Albacete, Albacete, Spain; Hospital Universitario Reina Sofia de Murcia, Murcia, Spain
Background: Carcinoma of the tongue is the predominant cancer type of the oral cavity. Human papillomavirus (HPV) has been detected in 99% of carcinomas from the uterine cervix and in many cases of head and neck carcinomas. The carcinogenic influence of genital HPVs in head and neck carcinoma development is controversial. We designed a study that analyzed the presence of HPV in squamous cell carcinomas (SCC) of the tongue and that determined the implicated genotypes. We compared these data with those obtained using the same methodology in uterine cervix dysplastic lesions of a population from the same geographic area.
Design: Clinical and histological data of 64 patients with SCC of the tongue were collected. Benign lesions of the tongue (30) and cervical dysplasia cases (332) were also processed in parallel to compare the HPV incidence and the genotypes of these lesions with those of carcinoma of the tongue. Paraffin blocks of all the cases were collected, and Polymerase Chain Reaction (PCR) was carried out using the SPF10 set of primers and the Innolypa genotyping methodology.
Results: HPV was detected in 26,2% of the tongue carcinoma cases. All positive cases showed high risk (HR) HPVs, being HPV 56 the most common (42,1%), followed by HPV 18 (26,3%), HPV 16 (10,5%), HPV 66 (10,5%), HPV 39 (5,3%), and HPV 51 (5,3%). None of the control cases of benign lesions of the tongue showed HR-HPV whereas 96,6% cases of cervical dysplastic lesions showed HR-HPV (HPV 16, 52, 51, 53, 31, 18, 33, and 58 were the genotypes detected in these cases).
Conclusions: - HPV incidence in squamous cell carcinoma of the tongue was 26,2%.
- The most prevalent HPV genotype was HPV 56, followed by HPV 18.
- HPV genotypes present in carcinomas of the tongue were markedly different from those found in dysplastic lesions of the cervix from a population of the same geographic area.
Category: Head & Neck
Wednesday, March 6, 2013 9:30 AM
Poster Session V # 204, Wednesday Morning