[1196] Stathmin Expression in Tumors from Various Organs – A Useful Biomarker for Confirming Endocervical Adenocarcinoma and Cervical Squamous Intraepithelial Lesions

Fan Lin, Jianhui Shi, Aihua Li, Taiying Chen, Haiyan Liu. Geisinger Medical Center, Danville, PA; Epitomics, Inc., Burlingame, CA

Background: Stathmin is a cytosolic microtubule destabilizing oncoprotein. The expression of stathmin in carcinomas from human organs has not been explored. We investigated the expression of stathmin in carcinomas and normal tissues from various organs.
Design: Immunohistochemical evaluation of stathmin (Epitomics; EP247) expression on 1,095 cases of carcinomas on tissue mircroarray sections and 154 biopsy specimens (Tables 1 and 2) of endocervical adenocarcinoma in situ (AIS) and invasive endocervical adenocarcinoma (IECA), high- and low-grade squamous intraepithelial lesions (HGSIL and LGSIL), benign squamous mucosa (BSM) and benign endocervical mucosa (BECG) was performed. The results (other than squamous dysplasia) were recorded as negative, 1+ to 4+. The stathmin expression on BSM, LGSIL and HGSIL was recorded as: 1+ (basal 2-3 layers stained); 2+ (bottom 2/3 stained); or 3+ (full thickness stained). Cytoplasmic staining with or without nuclear staining were regarded as positive.
Results: The positive staining results of carcinomas from various organs were as follows: germ cell tumors (64 of 64, 100%), urothelial CA (47 of 47, 100%), lung ADC (45 of 61, 74%), lung SCC (47 of 47, 100%), colonic ADC (62 of 64, 97%), breast CA (116 of 127, 91%), endometrial CA (131 of 131, 100%), ovarian serous CA (40 of 41, 98%), and other carcinomas (182 of 513, 35.5%). The stain in the normal tissues adjacent to these carcinomas was only focally and weakly positive. The results for cervical and endocervical lesions are summarized in Tables 1 and 2.

Table 1. Summary of immunostaining results on BECG, AIS and IECA
DiagnosisNegative1+2+3+4+Total positive cases (%)
BECG (N=24)2220009%
AIS (N=11)000110100%
IECA (N=31)032026100%

Table 2. Summary of immunostaining results on BSM, LGSIL and HGSIL
DiagnosisNegative1+2+3+Total positive cases (2+ and 3+)
BSM (N=20)020000
LGSIL (N=34)0921425 (74%)
HGSIL (N=17)0043034 (100%)

Conclusions: These data demonstrate that stathmin 1) is useful marker in differentiating benign endocervical glands from endocervical adenocarcinoma and in confirming the diagnosis of HGSIL and the majority of LGSIL; and 2) is a potential useful marker for confirming the diagnosis of urothelial carcinoma, endometrial carcinoma, ovarian serous carcinoma and lung squamous cell carcinoma, which is currently under investigation.
Category: Gynecologic & Obstetrics

Tuesday, March 5, 2013 1:00 PM

Poster Session IV # 229, Tuesday Afternoon


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