miroRNAs Involved in Development and Progression of Ovarian Serous Tumor
Sangho Lee, Jeonghyeon Lee, Seojin Kim, Insun Kim. Gacheon Gil Hospital, Incheon, Republic of Korea; Korea University Hospital, Seoul, Republic of Korea
Background: To define the role of microRNAs (miRNAs) in development and progression of ovarian serous tumor.
Design: The possible candidate miRNAs were selected from miRNA Chip study and verified using RT-PCR in 10 normal, 33 benign (SAD), 24 borderline (SBT), and 90 malignant tumor (SCA) tissues. The expressions of miRNAs were also evaluated with known clinicopathological parameters and patient's survival.
Results: The development of serous tumor from normal ovarian tissue was associated with the increase of miR-200b and miR-200c, but the decrease of miR-483, miR-125a and miR-146b. In SBT, miR-21, miR-210, miR-34a, miR-200b, and miR-200c were up-regulated than in SAD, whereas miR-483 and miR-30d were down-regulated. With progression from SBT to low grade SCA, miR-483 was up-regulated, whereas miR-34a was down-regulated in progression from low grade to high grade SCA. The up-regulation of miR-210, miR-483, miR-146b, miR-303, miR200b and miR-200c were associated with high FIGO and Silverberg grades, and clinical stage, whereas miR-34a was inversely correlated with high grade and high p53 expression. Up-regulation of miR-483 was associated with poor overall patient's survival.
Conclusions: Different miRNAs seem to play the role in development and progression of ovarian serous tumor and have prognostic significance.
Category: Gynecologic & Obstetrics
Wednesday, March 6, 2013 9:30 AM
Poster Session V # 176, Wednesday Morning