Metabolomic Profile of Endometriod Adenocarcinoma of the Endometrium
Sonia Gatius, Mariona Jove, Andree Yeramian, Maria Santacana, Judit Pallares, Manuel Portero, Reinald Pamplona, Xavier Matias-Guiu. Hospital Universitari Arnau de Vilanova, University of Lleida, IRB Lleida, Lleida, Spain
Background: Cancer cells need to change their metabolism for proliferation. Metabolomics have been used to identify individual metabolites which could be end points in molecular pathways affected in cancer cells.
Design: Fresh frozen tissue corresponding to normal endometrium in different phases of the menstrual cycle (25 cases) and endometrioid carcinoma, from surface (22 cases) and from myometrial invasive front (24 cases) were used for the metabolomics analysis. After metabolites extraction, samples were subjected to liquid chromatography coupled to mass spectrometry (HPLC-ESI-QTOF MS/MS) and analysed by both univariate and multivariate statistics.
Results: Multivariate analysis, used for pattern recognition, revealed differences between the three groups (normal endometrium, carcinoma from surface and carcinoma from myometrial invasive front). When tumoral vs. non tumoral samples were compared (T-Student, p<0.05), 54 differential molecules were found, three of them related to lipid metabolism. Interestingly, 112 different molecules (paired T-Student, p<0.05) were found between surface and myometrial invasive front samples involving changes in purine and tyrosine metabolism, among others.
Conclusions: The present results demonstrate different metabolomic pattern between normal endometrium and endometriod carcinoma, but also in endometrioid carcinoma with different levels of invasion. Such information is important to understand the molecular pathways involved in endometrioid carcinoma of the endometrium development and progression.
Category: Gynecologic & Obstetrics
Tuesday, March 5, 2013 9:30 AM
Poster Session III # 112, Tuesday Morning