MicroRNAs Expression Varies with Stromal Signatures in Myoinvasive Endometrioid Endometrial Carcinomas
Inigo Espinosa, Lluis Catasus, Irene Ferrer, Emanuela D'Angelo, Ana Mozos, Elena Serrano, Jaime Prat. Hospital de la Santa Creu i Sant Pau, Institute of Biomedical Research (IIB Sant Pau), Autonomous University of Barcelona, Barcelona, Spain
Background: The tumor microenvironment reflects the interaction between cancer cells and host stromal cells including fibroblasts, endothelial cells, and inflammatory cells. We have previously described several robust stromal response patterns that vary across myoinvasive endometrioid carcinomas. The macrophage (CSF1) signature was associated with higher tumor grade, lymphovascular invasion, and PIK3CA mutations. MicroRNAs (miRNAs) are post-transcriptional regulators involved in several biological processes and their deregulation is implicated in cancer. MicroRNA expression patterns may be of value for prognostic determinations as well as for eventual therapeutic intervention. In this study, we explore whether miRNAs expression varies with stromal signatures in myoinvasive endometrioid carcinomas.
Design: We performed comprehensive analysis of miRNA expression profiles (GeneChip® miRNA Array 2.0 Affymetrix) of 6 normal endometria and 24 endometrioid carcinomas in which the stromal signatures had been previously studied both by immunohistochemistry and in situ hybridization. Then, we analyzed which miRNAs were differently expressed by the different stromal signatures.
Results: There were distinct miRNA expression profiles for each stromal response as demonstrated by the unsupervised hierarchical clustering. Some miRNAs were differentially expressed in each stromal response indicating their possible functional role in this subset of tumors.
Conclusions: Our data show that different stromal responses have distinct miRNA expression patterns. The identification of unique miRNA signatures in each tumor type may have important clinical applications. The finding of some miRNAs differentially expressed in each stromal signature suggests their implication in tumor microenviroment and progression.
Category: Gynecologic & Obstetrics
Wednesday, March 6, 2013 1:00 PM
Poster Session VI # 202, Wednesday Afternoon