GATA3 Expression in Matched Paired Primary and Metastatic Urothelial Carcinomas
Lei Zhao, Gladell P Paner, Jerome B Taxy, Tatjana Antic, Maria Tretiakova. University of Chicago, Chicago, IL
Background: GATA3 has been recognized as a promising marker for primary urothelial carcinoma (UC), consistently showing higher expression levels than urothelial markers thrombomodulin and uroplakin III. However, the preservation of GATA3 expression in metastatic UC when compared to a primary site has not been investigated. The aim of this study was to evaluate the sensitivity of GATA3 as a diagnostic marker for metastatic UC by comparing expression of GATA3 in matched primary and metastatic conventional urothelial carcinomas, and to correlate it with well established UC-associated markers CK7 and p63.
Design: Tissue microarrays including 68 matched primary and metastatic UCs of conventional morphology were constructed, with normal urothelium as a control. Each case was represented by five 1 mm cores: 2 duplicate cores from the primary tumor and 2-3 cores from lymph node metastases. Immunohistochemical staining for GATA3, CK7 and p63 was performed with appropriate controls.
Results: Strong nuclear reactivity with GATA3 was observed in normal urothelium, 88.2% of primary UC and 91.2% of metastatic UC, showing similar sensitivity when compared to CK7 and significantly higher sensitivity when compared to p63 (p<0.05).
|Marker||Primary UC||Metastatic UC|
|GATA3||88.2% (60/68)||91.2% (62/68)|
|CK7||86.8% (59/68)||88.2% (60/68)|
|p63||73.5% (50/68)||76.5% (52/68)|