Immunohistochemical Expression of Maspin, p53 and Ki67 in Bladder Urothelial Tumors, and Their Relationship with Clinicopathological Parameters*
Gulcan Yavuz, Sema Bircan. Suleyman Demirel University School of Medicine, Isparta, Turkey
Background: Maspin is a member of the serpin (serin protease inhibitor) family and has been shown to be a suppressor of tumor growth. In this study, we aimed to investigate maspin, p53 and Ki67 expressions in bladder urothelial tumors immunohistochemically, and to examine their relationship with clinicopathologic parameters.
Design: We examined 84 bladder urothelial tumors and 10 nontumoral bladder tissues. Urothelial tumors were classified according to the 2004 World Health Organisation. pT stages of all cases were categorized as noninvasive (pTa), lamina propria invasive (pT1) and muscularis propria invasive cases (pT2+). Immunohistochemistry was performed on tissue microarray (TMA) sections for maspin, p53 and Ki67 antibodies.
Results: This study included 6 (7.1%) papillary urothelial neoplasm of low malignant potential (PUNLMP), 38 (45.2%) low grade (LG) and 40 (47.6%) high grade (HG) carcinomas. Thirty (35.7%), 17 (20.2%) and 37 (44%) cases had pTa, pT1 and pT2+ stages, respectively. Maspin expression was found in 3 (30%) of nontumoral cases and 53 (63,1%) of urothelial tumor, which was significantly higher in tumoral cases (p=0.048). It was significantly higher in pTa (73.3%) and pT1 (76.5%) groups than pT2+ muscle invasive cases (48.6%) (p=0.050). Eight (%9,5), 21 (%25) and 24 (%28,6) urothelial tumor showed +1, +2 and +3 maspin immunostaining level, respectively. The level of maspin expression inversely correlated with pT stages of the tumors (p=0.012, r=-0.274), and it was tend to be inversely correlated with histological grade (p=0.071, r=-0.198). P53 and Ki67 expressions were detected in 20 (23.8%) and 50 (59.5%) of tumoral cases, respectively. P53 expression was significantly higher in HG and pT2+ muscle invasive cases (p<0.0001, p<0.0001, respectively), and Ki67 expression was also tended to be higher in HG and pT2+ cases (p=0.051, p=0.057, respectively). Furthermore, maspin expression showed a significant inverse correlation with p53 expression (p=0.015).
Conclusions: According to our results, maspin expression is decreased with increasing grade and pT stages of urothelial tumors. In contrast, p53 expression seems to be correlated with high grade and invasive stage of the tumors. Our results suggest that maspin expression may play an important role in the progression of urothelial tumors, and its downregulation together with upregulation of p53 probably facilitates invasion in bladder urothelial carcinomas.
*This study was supported by Suleyman Demirel University Scientific Research Projects Coordination Unit.
Category: Genitourinary (including renal tumors)
Wednesday, March 6, 2013 1:00 PM
Poster Session VI # 166, Wednesday Afternoon