[1073] SF-1 Immunohistochemical Expression in Tumors

Myra Wilkerson, Fan Lin, Jianhiu Shi. Geisinger Medical Center, Danville, PA

Background: Steroidogenic factor 1 (SF-1) is a nuclear receptor that functions with other transcription factors to regulate several genes in the hypothalamic-pituitary-gonadal axis, and in gonadal and adrenal steroidogenesis. SF-1 is required for regulation of sex determination and differentiation, reproduction, and steroidogenesis. Nuclear expression of SF-1 has been described in adrenal cortical neoplasms and normal adrenal cortex, endometriosis, Leydig cells, Sertoli cells, and theca and granulosa cells. Published data on SF-1 expression in tumors is limited. In this study, we investigated the expression of SF-1 in a large series of tumors from various organs.
Design: Immunohistochemical evaluation of SF-1 expression (R&D systems, SF-1/NR5A1 antibody, clone N1665) was performed on 984 tumors from various organs using tissue microarray sections. The staining distribution was recorded as negative (<5% of tumor cells stained), 1+ (5-25%), 2+ (26-50%), 3+ (51-75%), or 4+ (>75%).
Results: The positive staining results (%) and the total number of cases for each entity (N) are summarized in Table 1. Twenty-three of 984 cases demonstrated positive nuclear staining for SF-1 protein and these cases consisted of adrenal cortical adenomas and testicular Leydig cell tumors. In addition, benign interstitial Leydig cells and Sertoli cells in seminiferous tubules evaluated for ITGCN all demonstrated positive staining for SF-1.

Table 1. Summary of SF-1 immunostaining in 998 cases
TumorN# Positive% Positive
Adrenal cortical adenoma242083.3
Testicular Leydig cell tumor33100
Testicular ITGCN15  
Testicular classic seminoma21  
Testicular embryonal CA18  
Testicular yolk sac tumor6  
Urothelial CA primary to bladder45  
Pulmonary neuroendocrine CA26  
Pulmonary ADC52  
Pulmonary squamous cell CA40  
Pancreatic endocrine CA16  
Clear renal cell CA68  
Papillary renal cell CA17  
Chromophobe renal cell CA23  
Infiltrating duct CA of breast94  
Infiltrating lobular CA of breast26  
Esophgeal ADC26  
Gastric ADC12  
Colonic ADC28  
Hepatocellular CA17  
Papillary CA of thyroid42  
Follicular CA of thyroid30  
Follicular adenoma of thyroid51  
Prostatic ADC87  
Ovarian serous ADC43  
Endocervical ADC22  
Endometrial ADC132  
Total tumors984232.3
ADC-adenocarcinoma; CA-carcinoma

Conclusions: Our data demonstrate that SF-1 is a highly sensitive and specific marker for tumors of steroid producing cells, including adrenal cortical adenomas and Leydig cell tumors. In addition, it can be used as a marker for normal Leydig and Sertoli cells. SF-1 does not appear to be expressed in testicular germ cell tumors, or in tumors from other organs.
Category: Genitourinary (including renal tumors)

Wednesday, March 6, 2013 1:00 PM

Poster Session VI # 181, Wednesday Afternoon


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