[1059] GATA3 Expression Alteration in High Grade and High Stage Urothelial Carcinoma

Celina Villa, Bing Zhu, Chunyan Luan, Ximing J Yang. Northwestern University, Chicago, IL

Background: GATA3 has been found to be a sensitive marker for urothelial carcinoma, breast carcinoma and transitional proliferations of the female genital tract. The specific role of GATA3 expression in high grade urothelial carcinomas including the micropapillary variant of urothelial carcinoma and with respect to pathologic stage has not been reported. We sought to investigate whether GATA3 expression is retained in high grade lesions of urothelial carcinoma and if any correlation exists with respect to pathologic stage.
Design: Urothelial carcinomas queried from the Surgical Pathology archives at Northwestern University were examined by immunohistochemistry for GATA3 expression. Immunostaining was carried out with a GATA3 specific monoclonal antibody. Nuclear staining for GATA3 was graded as negative (absent or weak expression, <5% of carcinoma cells) and positive (moderate or strong expression, >5% of carcinoma cells) with focal (5-50% of carcinoma cells) or diffuse (>50% of carcinoma cells) pattern. GATA3 expression was analyzed with respect to grade of tumor (low versus high) and pathologic stage (Results: A total of 92 urothelial carcinomas were analyzed including 19 micropapillary variants, 57 high grade urothelial carcinoma and 16 low grade urothelial carcinomas. Diffuse, positive GATA3 expression was seen in 100% (19/19) of micropapillary variants, 88% (14/16) of low grade urothelial carcinomas and 77% (44/57) of high grade urothelial carcinoma. Loss of GATA3 expression was significantly associated with higher pathologic stage (T ≥2 in 13/58) compared with lower stage (T<2 in 1/34) (p<0.05).
Conclusions: Our results indicate that GATA3 immunoreactivity does not change significantly in micropapillary and high grade variants of urothelial carcinoma. However, decrease or loss of GATA3 expression was seen more frequently in tumors with pathologic stage of T ≥2. The findings of retained GATA3 expression in micropapillary urothelial carcinoma, which often shows a loss of expression of other urothelial markers such as p63, may be helpful for determining the origin of micropapillary carcinoma of unknown primary.
Category: Genitourinary (including renal tumors)

Tuesday, March 5, 2013 9:30 AM

Poster Session III # 105, Tuesday Morning

 

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