Expression of Syndecan-1 (CD138) in Bladder Cancer with Emphasis on Conventional Urothelial Carcinoma and Urothelial Carcinoma with Squamous Differentiation
Samip Patel, Adeboye O Osunkoya, Claudia Ormenisan, Gabriela M Oprea-Ilies. Emory University, Atlanta, GA
Background: Syndecan-1(CD138) is a member of the family transmembrane heparan sulfate proteoglycans, which are involved in cell-to-cell adhesion and the interaction of cells with the extracellular matrix, cell migration and angiogenesis. Altered CD138 (Syndecan-1) expression has been described in carcinogenesis of various tumors in which it correlates with gain of malignant characteristics and adverse outcome. Syndecan-1(CD138) expression has also been described in Plasmacytoid variant of Urothelial Carcinoma (UCa). However, Syndecan-1(CD138) expression has not been well characterized in conventional UCa and UCa with squamous differentiation.
Design: Tissue microarrays (TMA) were constructed from 155 bladder tumor specimens retrieved from 82 patients. 40/155 cases (26%) demonstrated at least focal squamous differentiation. Immunohistochemical stains for Syndecan-1(CD138) was performed on the TMAs, and intensity, percentage, and pattern of staining in tumor cells was documented. Data was recorded with respect to tumor type, grade, stage, patient age, gender, and clinical outcome when available (39 cases). Preliminary statistical analysis was performed by Chi-square analysis. Kaplan-Meier survival plots were constructed, and disease-free survival was compared via log-rank test.
Results: There was a statistically significant correlation between cytoplasmic granular staining and UCa with squamous differentiation by chi-square (p<0.001), with a kappa statistic of 0.686 (p<0.001). 3+ cytoplasmic granular staining was associated with non-papillary architecture (p=0.001), high T-stage (p=0.02), and high M-stage (p=0.02) but not with tumor grade, patient age, or gender (p>0.05).
Conclusions: 1. UCa with squamous differentiation demonstrates statistically significantly increased cytoplasmic granular expression of Syndecan-1(CD138) relative to conventional UCa. Syndecan-1(CD138) may be involved in the pathogenesis of squamous differentiation in UCa, and may conceivably play a role in the aggressive phenotype in this variant of UCa.
2. Anti-Cd138 agents may be useful in treating these more aggressive urothelial tumors.
Category: Genitourinary (including renal tumors)
Tuesday, March 20, 2012 1:00 PM
Poster Session IV # 92, Tuesday Afternoon