The Epidermal Growth Factor Receptor (EGFR) Is Frequently Overexpressed in Penile Squamous Carcinomas
George J Netto, Antonio L Cubilla, Betina Katz, Kristen L Lecksell, Rajni Sharma, Alcides Chaux. Johns Hopkins University, Baltimore, MD; Instituto de Patologia e Investigacion, Asuncion, Paraguay
Background: Overexpression of the epidermal growth factor receptor (EGFR) has been described in a variety of tumors but experience in penile squamous cell carcinomas (SCC) is scant. Current adjuvant and neoadjuvant treatment options for penile cancer can be improved by the identification of new targets of therapy. Herein, we evaluated EGFR expression in a large set of penile SCC.
Design: EGFR immunoexpression was evaluated in 112 cases of penile SCC, from which 4 tissue microarrays (TMA) were built. TMA spots were scanned using the APERIO system and uploaded to the TMAJ platform (http://tmaj.pathology.jhmi.edu). Scanned images were digitally-analyzed with ImageJ (http://rsbweb.nih.gov/ij) and the immunomembrane plug-in (http://imtmicroscope.uta.fi/immunomembrane). Staining completeness (0–10 points) and intensity (0–10 points) were added for a combined score (0–20 points). Cases were classified as: EGFR negative, 0–3 points; low expression, 4–8 points; high expression, 9–20 points.
Results: 50 cases (45%) showed high EGFR expression, 49 cases (44%) demonstrated low EGFR expression, and 13 cases (11%) were negative. No association was observed with histologic subtype (Fisher's P = .55) or histologic grade (Fisher's P = .62).
Conclusions: EGFR is frequently overexpressed in penile SCC, with high levels in almost one half of the cases. EGFR expression is not related to histological subtype or grade. These results suggest a potential role for EGFR inhibitors in the treatment of patients with penile carcinomas.
Category: Genitourinary (including renal tumors)
Monday, March 19, 2012 1:00 PM
Poster Session II # 147, Monday Afternoon