p16 Expression Is Not Associated with Human Papillomavirus (HPV) in Urinary Bladder Squamous Cell Carcinoma
Jennifer B Kum, Yingchuan Hu, Rodolfo Montironi, Antonio Lopez-Beltran, Guorong Chen, Gregory T MacLennan, Muhammad T Idrees, Thomas M Ulbright, David G Grignon, John N Eble, Liang Cheng. Indiana University School of Medicine, Indianapolis, IN; Polytechnic Univeristy of the Marche Region (Ancona) United Hospitals, Ancona, Italy; Cordoba University, Cordoba, Spain; First Affiliated Hospital of Wenzhou Medical College, Wenzhou, China; Case Western Reserve University, Cleveland, OH
Background: Squamous cell carcinoma of the urinary bladder is an unusual histologic type of bladder neoplasm with unknown etiology. There is a well established association between human papillomavirus (HPV) infection and the development of uterine cervical and head/neck squamous cell carcinomas. However, the role of HPV in the pathogenesis of squamous cell carcinoma of the urinary bladder is uncertain. The purposes of this study are to investigate the potential role of HPV in the development of squamous cell carcinoma of the urinary bladder and to determine if p16 expression is a surrogate marker for HPV in this malignancy.
Design: Twenty-three cases of squamous cell carcinoma of the urinary bladder and 15 cases of urothelial carcinoma with squamous differentiation are included in this study. HPV infection is analyzed by both in situ hybridization at the DNA level and immunohistochemistry at the protein level. p16 protein expression is analyzed by immunohistochemistry.
Results: HPV DNA and protein are not detected in 23 cases of squamous cell carcinoma (0%, 0/23) and 15 cases of urothelial carcinoma with squamous differentiation (0%, 0/15). p16 expression is detected in 8 cases (35%, 8/23) of squamous cell carcinoma and 6 cases (40%, 6/15) of urothelial carcinoma with squamous differentiation. There is no correlation between p16 expression and the presence of HPV infection in bladder squamous cell carcinoma and urothelial carcinoma with squamous differentiation.
Conclusions: HPV does not play a role in the development of squamous cell carcinoma of the urinary bladder and urothelial carcinoma with squamous differentiation. p16 expression should not be used as a surrogate marker for evidence of HPV infection in either squamous cell carcinoma of the urinary bladder or urothelial carcinoma with squamous differentiation as neither HPV DNA nor protein is detectable in these neoplasms.
Category: Genitourinary (including renal tumors)
Wednesday, March 21, 2012 1:00 PM
Poster Session VI # 179, Wednesday Afternoon