MicroRNA Profiles in Osteosarcoma
Kyu Yeoun Won, Youn Wha Kim, Yong-Koo Park. Kyung Hee University Hospital at Gangdong, College of Medicine, Kyung Hee University, Seoul, Republic of Korea; Kyung Hee Medical Center, College of Medicine, Kyung Hee University, Seoul, Republic of Korea
Background: microRNAs (miRNA) are evolutionarily conserved small non-coding RNAs, which regulate gene expression by translational inhibition or cleavage of target mRNAs. The miRNAs play important roles in the development, differentiation and fuction of various cell types, and in the pathogenesis of various human diseases. The miRNAs are differentially expressed in normal cells and cancer cells.
Design: We performed a miRNA microarray analysis on 8 formalin fixed paraffin embedded osteosarcoma tissue samples and two osteosarcoma cell lines (SJSA-1 and MG63). And we confirmed the result of microarray by reverse transcription polymerase chain reaction.
Results: The data from miRNA profiling of osteosarcoma indicated that 10 miRNAs showed 10-fold increased expression compared to normal control. Among the 10 miRNAs, three miRNAs (miR-199b-5p, miR-338-3p, and miR-891a) were confirmed with reverse transcription polymerase chain reaction. The miR-199b-5p, miR-338-3p, and miR-891a were increased by 13.6-fold, 6.2-fold, and 6.8-fold respectively.
Conclusions: The profiles of miRNAs in the osteosarcoma is largely unknown. The investigation of the miRNAs expression between normal and osteosarcoma is crucial step for future clinical trials. The miRNAs have been recently shown to be useful tools to silence cancers. The miR-199b-5p is known to be involved in Notch pathway, targeting HES1. And miR-199b-5p expression is low in metastatic medulloblastoma patients. And miR-199b-5p expression specifically impairs the caner-stem-cell population, which results in impairment of medulloblastoma tumor development in the cerebellum xenograft mouse model. Recent report showed that miR-338-3p contribute to the formation of basolateral polarity in epithelial cells. These results revealed a potentially important role for miRNAs in the process of epithelial cell differentiation. The disruption of polarity in epithelial cells is associated with poor prognosis for carcinomas. However, in mesenchymal tumor such as osteosarcoma, the miRNAs function is largely unknown. The miR-891a is known to be associated with BLCAP (bladder cancer associated protein). This gene encodes a tumor suppressor protein that reduces cell growth by stimulating apoptosis. Further studies about the specific function of three miRNAs in osteosarcomas is needed.
Category: Bone & Soft Tissue
Tuesday, March 20, 2012 9:30 AM
Poster Session III # 4, Tuesday Morning