Immunohistochemical Profile of Renal Cell Carcinoma in Patients Younger than 45 Years of Age: Analysis of 87 Cases of Different Tumor Subtypes
Ryan Carr, Guoping Cai, Yunhua Bao, Manju L Prasad, Satish K Tickoo, Adebowale J Adeniran. Yale University School of Medicine, New Haven, CT
Background: Renal Cell Carcinoma (RCC) is distinctly rare in children and young adults. Because of the rarity of the tumor in this age group, the immunohistochemical profile has not been fully described. The goal of this study is to define the immunohistochemical profile of the various subtypes of RCC in patients aged 45 and younger.
Design: A tissue microarray block with 87 cases of RCC (49 clear cell [CRCC], 23 papillary (PRCC), 10 Chromophobe [ChRCC] and 5 translocation-associated [TxRCC]) was constructed. The tumors were stained with the following diagnostic antibodies: cytokeratin (CK) AE1/AE3, CK7, CK903, TFE3, TFEB, CD10, AMACR, C-KIT, Vimentin, EMA, CA-IX; and with the following prognostic antibodies: PAX-8, survivin and VHL. The slides were reviewed and a stain was deemed to be positive if it demonstrated moderate to strong intensity in more than 5% of the tumor cells.
Results: Results of the immunohistohistochemical stains are listed in Table 1.
|Tumor type||Immunohistochemical profile|
|CRCC||AE1/AE3, Vimentin, CD10, EMA, CA-IX positive; PAX-8 variable; C-KIT, CK903 negative|
|PRCC||AE1/AE3, AMACR, Vimentin, CK7, EMA, PAX-8 positive; C-KIT, CA-IX, negative|
|ChRCC||AE1/AE3, CD10, C-KIT, CK7, EMA positive; AMACR, Vimentin, CK903, CA-IX, PAX-8 negative|
|TxRCC||TFE3, CD10, Vimentin positive; AE1/AE3, C-KIT, CK903, CK7, CA-IX negative|