The Prognostic Relevance of Nuclear Factor-κB Activation in Papillary Thyroid Carcinoma
Jung-Soo Pyo, Guhyun Kang, Dong-Hoon Kim, Seoung Wan Chae, Jin Hee Sohn. Kangbuk Samsung Hospital, Sungkyunkwan University School of Medicine, Seoul, Korea
Background: Although the studies of papillary thyroid carcinoma (PTC) have been focused on the RAS-RAF-MEK-ERK-MAP kinase pathway, the majority of other signaling pathways possibly involved in the tumorigenesis of PTCs have not been fully elucidated. In this study, we investigated the nuclear factor-κB (NF-κB) signaling pathway in PTC using morphoproteomics and correlated the results with clinicopathological parameters.
Design: Immunohistochemistry for NF-κB RelA was performed on archival paraffin-embedded tissues of PTC obtained from 138 patients, and subcellular compartmentalization (nuclear and cytoplasmic) was evaluated.
Results: Nuclear expression of NF-κB RelA regardless of cytoplasmic expression was identified in 94 of 125 PTCs (75.2 %), and was more frequent in PTCs larger than 1 cm (P < 0.001). The nuclear expression was not observed in nonneoplastic nodules and benign follicular neoplasms of the thyroid. There were significant differences in the clinicopathological parameters, such as extrathyroidal extension (P = 0.013), nodal metastasis (P = 0.020), BRAFV600E mutation (P = 0.033), cyclin D1 overepxression (P < 0.001) and Ki-67 labeling index (P = 0.042) between the groups with and without nuclear expression of NF-κB. However, BRAFV600E mutation status and cyclin D1 overexpression was not correlated with any clinicopathological variables.
Conclusions: Our results provide the evidence of activation of NF-κB signaling pathway in PTCs. The expression pattern of NF-κB may serve as a prognostic marker and a potential therapeutic target.
Tuesday, March 20, 2012 1:00 PM
Poster Session IV # 34, Tuesday Afternoon