Expression of Lymphatic-Specific Markers in Vascular Malformations
Arun Gopinath, Heh Shin Kwak, Paula E North, Soheil S Dadras. Hartford Hospital, Hartford, CT; Knott Street Dermatology, Portland, OR; Medical College of Wisconsin, Milwaukee, WI; University of Connecticut School of Medicine, Farmington, CT
Background: Vascular malformations (VM) are a heterogenous group of localized defects in vascular morphogenesis, composed of varying degree of lymphatic and/or vascular endothelium. Distinguishing lymphatic from vascular endothelial cells on routine histology can be very challenging.
Design: We sought to identify the degree of lymphatic and vascular components of VMs (n=32) by immunostaining for Prox-1, Podoplanin (D2-40), LYVE-1 and CD31. The test cases included paraffin-embedded tissues sections from lesions with lymphatic morphology: microcystic lymphatic malformation (n=7), mixed micro and macrocystic lymphatic malformation (n=7) and venous lymphatic malformation (n=10); and lesions with vascular morphology: venous capillary malformation (n=2) and Port-wine stains (n=6). were analyzed. Based on the degree of marker expression by the lesional cells, a semi-quantitative immunoscore was assigned as follows: 0, no expression; 1, <10%; 2, 11-50%; and 3, >51%.
Results: Lesions with lymphatic morphology consistently expressed both lymphatic and vascular markers whereas those with vascular morphology were negative for these lymphatic markers.
Conclusions: Immunostaining for lymphatic and vascular markers can be helpful in determining the degree of lymphatic and vascular endothelium in difficult to diagnose cases of VMs.
Tuesday, March 20, 2012 9:30 AM
Poster Session III # 93, Tuesday Morning