[461] Frequency and Distribution of 40 HPV Genotypes in Uninsured Latino Women with Abnormal Pap Tests

Haijun Zhou, Dina R Mody, Mary R Schwartz, Christopher D Hobday, Debora Smith, Sonia R Hodgson, Donna Coffey, Yimin Ge. The Methodist Hospital, Houston, TX; Weill Medical College of Cornell University, Houston

Background: Knowledge about the prevalence and distribution of HPV genotypes in cervical premalignant and malignant lesions is crucial to guide development of clinical management strategies and of prophylactic vaccines. The aim of this study was to determine the frequency and distribution of HPV genotypes in an underserved cohort of women.
Design: From 1/2010 to 4/2011, 808 SurePath cervical specimens were collected from uninsured Latino women who were referred to our institution for abnormal Pap tests. The patients' average age was 36.5 years (range 19-85 years). The specimens were tested for 40 HPV genotypes by DNA microarray and sequencing assay.
Results: In this underserved cohort of women, the HPV infection rate was high with frequent multi-strain infection (38.4%). The combined frequency of HPV16/18 was 55.1% in HSIL. HPV6 and 11 were infrequent (2.9% and 1.1%). The frequency of HPV 90 was unexpectedly high (2.4%) and associated with dysplasia (9.4%).
Conclusions: The frequency and distribution pattern of HPV genotypes in this cohort differs from previously published US data. HPV 90, an uncommon genotype in the US was identified in the cohort. Understanding the differences and possible changes in HPV distribution pattern may help guide the development of appropriate preventative and therapeutic strategies targeting underserved population.

Table 1. Frequencies and Distribution of 40 HPV Genotypes in Major Cytology Categories

Cytology Diagnosis	No HPV(%)	LR-HPV% (S/M)*	IR-HPV% (S/M)	HR-HPV% (S/M)	Total HPV infection %	Most frequent HR-HPV types (in decreasing order)
NILM (n=497)	9.5	28 (37/23)	13 (15/12)	58 (48/64)	90.5	16, 18, 53, 52, 39, 45, 66, 67, 90, 56
ASCUS (n=48)	8.3	28 (32/27)	8 (0/9)	64 (68/62)	91.7	16, 18, 45, 51, 52, 53, 42, 35, 59, 67
ASC-H (n=9)	11	0 (0/0)	33 (20/43)	67 (80/57)	89	31, 53, 18, 39, 52, 58, 66
LSIL (n=167)	1.2	24 (23/24)	18 (17/18)	59 (60/58)	98.8	53, 56, 16, 18, 66, 58, 39, 51, 67, 82
LSIL-H (n=25)	0	15 (0/24)	6 (11/3)	78 (89/72)	100	16, 58, 51, 45, 39, 31, 53, 67, 18, 33
HSIL (n=56)	1.8	18 (10/22)	5 (0/7)	77 (90/69)	98.2	16, 31, 18, 45, 39,56, 58, 59, 53, 43
Total‡	7.1	26 (31/24)	13 (13/13)	62 (56/63)	92.9	16, 18, 53, 56, 39, 58, 45, 52, 66, 67

*Percentage of HPV infection (Single strain infection/Multi-stain infection); LR-HPV, IR-HPV and HR-HPV represent low, intermediate and high risk HPV, respectively. ‡ include rare AGC (2) and cancer (1) cases (not shown).


Category: Cytopathology

Monday, March 19, 2012 9:30 AM

Poster Session I Stowell-Orbison/Surgical Pathology/Autopsy Awards Poster Session # 54, Monday Morning