[432] Utility of Fine Needle Aspiration for c-MYC Interphase Fluorescence In-Situ Hybridization Analysis of Aggressive B-Cell Lymphomas

Sarwat W Siddiqui, Kieron Dunleavy, Diane C Arthur, Armando C Filie. National Cancer Institute, Bethesda, MD

Background: c-MYC translocation is characteristically seen in Burkitt lymphomas and in approximately 10% of diffuse large B-cell lymphomas, not otherwise specified (DLBCL). In this study we report our experience on performing interphase fluorescence in-situ hybridization (FISH) analysis for c-MYC rearrangement on fine needle aspirates (FNA) of Burkitt lymphoma and DLBCL.
Design: We retrieved from our files 17 superficial/image-guided FNA cases of Burkitt lymphoma and DLBCL that had concomitant interphase FISH analysis performed for c-MYC rearrangement. Smears and/or cytospins were reviewed for sample cellularity and correlated with the results of the interphase FISH analyses.
Results: Interphase FISH analysis provided an informative results in 14 (82%) of the 17 cases (See table below). Most of these cases showed moderate to high cellularity. Only one case was paucicellular, however, the atypical lymphoid cells were intact and preserved. Three cases (18%) were non-informative for c-MYC status. On review, all three cases were paucicellular. One of these cases showed mostly degenerated cells with only rare intact atypical lymphoid cells. The remaining two cases showed primarily blood and no diagnostic atypical lymphoid cells.

c-MYC interphase FISH
Moderate to high490

Conclusions: FNA is a minimally invasive technique that provides sufficient and good quality material for c-MYC interphase analysis of aggressive B-cell lymphomas.
Category: Cytopathology

Wednesday, March 21, 2012 1:00 PM

Poster Session VI # 84, Wednesday Afternoon


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