Utility of Fine Needle Aspiration for c-MYC Interphase Fluorescence In-Situ Hybridization Analysis of Aggressive B-Cell Lymphomas
Sarwat W Siddiqui, Kieron Dunleavy, Diane C Arthur, Armando C Filie. National Cancer Institute, Bethesda, MD
Background: c-MYC translocation is characteristically seen in Burkitt lymphomas and in approximately 10% of diffuse large B-cell lymphomas, not otherwise specified (DLBCL). In this study we report our experience on performing interphase fluorescence in-situ hybridization (FISH) analysis for c-MYC rearrangement on fine needle aspirates (FNA) of Burkitt lymphoma and DLBCL.
Design: We retrieved from our files 17 superficial/image-guided FNA cases of Burkitt lymphoma and DLBCL that had concomitant interphase FISH analysis performed for c-MYC rearrangement. Smears and/or cytospins were reviewed for sample cellularity and correlated with the results of the interphase FISH analyses.
Results: Interphase FISH analysis provided an informative results in 14 (82%) of the 17 cases (See table below). Most of these cases showed moderate to high cellularity. Only one case was paucicellular, however, the atypical lymphoid cells were intact and preserved. Three cases (18%) were non-informative for c-MYC status. On review, all three cases were paucicellular. One of these cases showed mostly degenerated cells with only rare intact atypical lymphoid cells. The remaining two cases showed primarily blood and no diagnostic atypical lymphoid cells.
|Moderate to high||4||9||0|