Karyotyping Myofibroblastic/Fibroblastic Tumors: Continuing Usefulness of Standard Cytogenetic Methods in Detecting Novel Genetic Findings and Confirming the Histologic Diagnosis
Megan Fitzpatrick, Francine Leech, David Meredith, Robert Quinn, Therese Bocklage. University of New Mexico School of Medicine, Albuquerque, NM; UNM SOM, Albuquerque, NM
Background: Myofibroblastic/Fibroblastic tumors encompass a broad variety of benign to borderline to malignant tumors. The category continues to undergo change as tumors are more accurately classified. Because this is a large, diverse category, karyotypic information is limited. Classic cytogenetics is still helpful in providing first leads to uncover molecular level abnormalities. However, artifacts of long term in-situ culture must be recognized and threshed from the harvest of findings.
Design: Karyotypes of myofibroblastic/fibroblastic tumors (M-FT) according to W.H.O. 2002 classification and recently proposed M-FT were searched for in the cytogenetics database at our institution for 2001-2011 (to October 1). Twelve categories of tumors were identified. Tumors were stratified according to: 1) successful culture, 2) normal karyotype (possible fibroblast overgrowth), 3) abnormal clonal karyotype expected for the histologic diagnosis, and 4) novel abnormal karyotype. Percent successful karyotype per tumor type and percent non-clonal cell lines were calculated. Length in time in culture and type of specimen (biopsy, resection, re-exision) were noted in conjunction with tumor diagnosis, location, size, grade, and stage.
Results: The results are provided in table form.
|TUMOR||NUMBER||NUMBER SUCCESSFULLY KARYOTYPED||NUMBER WITH NORMAL KARYOTYPE||NUMBER WITH EXPECTED KARYOTYPE||NUMBER WITH NOVEL ABNORMAL KARYOTYPE||PERCENT WITH ABNORMAL KARYOTYPE|
|CALCIFYING APONEUROTIC FIBROMA||2||1||0||0||1||100%|
|FIBROSARCOMA ARISING IN DFSP||2||2||0||0||2||100%|
|INFLAMMATORY MYOFIBROBLASTIC TUMOR||2||2||1||1||0||50%|
|LOW GRADE FIBROMYXOID SARCOMA||9||7||2||2||3||71%|
|SCLEROSING EPITHELIOID FIBROSARCOMA||1||1||0||1||0||100%|
|MYOFIBROBLASTIC/FIBROBLASTIC TUMOR NOS||9||6||3||0||3||50%|