Establishing Optimal Digital Scanning Parameters of 3-D Gynecological Virtual Images: Follow-Up Study
Adam J Horn, Stanley J Radio, Maheswari S Mukherjee, Subodh M Lele, Najia Wright, Jane L Meza, Amber D Donnelly, Mary F McGaughey, Alicia M Bryan. School of Allied Health, University of Nebraska Medical Center, Omaha, NE; University of Nebraska Medical Center, Omaha, NE
Background: Virtual microscopy (VM) in cytology has been limited by the inability of focusing through the 3 dimensional (3D) cell clusters of cytological specimens acquired with single focal plane (2D images). Limited information exists regarding the optimal scanning parameters for 3D scanning. We previously established an optimal scanning interval of 1 micron. The goal of this study is to determine the optimal number of scanning focal plane levels.
Design: Twenty SurePath™ slides were scanned at 40X magnification at 1 micron with 7 focal planes (Group 1),5 focal planes (Group 2) and 3 focal planes (Group 3) using iScanCoreo Au scanner (Ventana, AZ, USA). 60 virtual images (VI) were produced.
A cytopathologist, cytotechnologist, pathology resident and a cytotechnology student used BioImagene's Image Viewer (version: 22.214.171.124 – RC8) and diagnosed pre-annotated cells of group 1, 2 and 3. They recorded the focal plane level at which they were confident of the diagnosis. Subsequently, they diagnosed the corresponding 20 glass slides (Group 4) using conventional Light Microscopy (LM). Participants selected standard Bethsda diagnostic categories. We evaluated interobserver reliability using kappa statistics.
Results: The interobserver reliability was found to be highest for the glass slides (97%) followed by VI scanned at 3 focal plane levels (80%), 7 focal plane levels (78%) and 5 focal plane levels (76%). See table 1.
Conclusions: VI scanned with 3 focal plane levels at 1 micron interval has potentially the highest interobserver reliability, sensitivity, NPV and the lowest file size. This finding will guide a larger confirmatory study to establish the optimal scanning focal plane and interval of gynecologic specimens.
Monday, March 19, 2012 9:30 AM
Poster Session I Stowell-Orbison/Surgical Pathology/Autopsy Awards Poster Session # 58, Monday Morning