The Prevalence of Common RNA Enteroviral Pathogens from Formalin-Fixed Paraffin-Embedded Myocardium Tissues of Myocarditis
Jie Yang Jhuang, Chih Kang Hong, Chia Tung Shun. National Taiwan University Hospital, Taipei, Taiwan; National Taiwan University, Taipei, Taiwan
Background: The etiological factor of sudden cardiac death due to acute myocarditis has long been an important issue in forensic medicine. Due to the progression of molecular technique, there are increasing researches about the etiology of myocarditis. Although the cause of myocarditis in any given pations, systemic diseases, drugs, and toxins have been associated with the development of this disease. Viruses are an important cause of myocarditis in North America and Europe. The prevalence of viral myocarditis is still unclear in Taiwan. The purpose of this study was to investigate the prevalence of myocarditis infected by RNA enterovirus in Taiwan.
Design: The formalin fixed paraffin embedded myocardial tissue blocks of myocarditis were obtained from endomyocardial biopsy (9 samples), heart transplantation (1sample) and forensic autopsy specimen die of myocarditis (1 sample). Tissue blocks were studied by using immunohistochemistry and reverse transcriptase-polymerase chain reaction (RT-PCR) for enterovirus. This study showed that histological sections from 7 of 11 myocarditis cases were positive for the viral capsid protein VP1 by immunohistochemical staining. In 4of 7 VP1 immunohistochemical positive staining specimens, viral genome of enterovirus was detected by RT-PCR using 5'NTR genomic fragment.
Results: These results showed most RNA fragment recovered by RT-PCR which fall between 152-470 nt. The product of RT-PCR probe should less than200 bp which is the best candidate in these recovery. Also the study provided data of rough enteroviral prevalence in myocarditis (more than 36.4%).
Conclusions: Further evidence of prevalence about enterovirus involvement in myocarditis needs more large scale of such cases using the method provided in the study to get in Taiwan.
Wednesday, March 21, 2012 1:00 PM
Poster Session VI # 37, Wednesday Afternoon