Identification of Heterogeneous Nuclear Ribonucleoprotein K (hnRNP K) as a Biomarker in Hepatocellular Carcinoma in Patients with Cirrhosis by Proteomic and Immunohistochemical Studies
Yantong Guo, Jingming Zhao, Jingtao Bi, Mingyi Chen. Beijing Jishuitan Hospital, Beijing University Health Science Center, Beijing, China; University of California Davis Medical Center, Sacramento, CA
Background: Hepatocellular carcinoma (HCC), one of the most common malignant tumors worldwide, is particularly prevalent in Asian countries. Hepatitis B (HBV) and Hepatitis C (HCV)-associated cirrhosis are the major common risk factors of HCC. The diagnosis of HCC in a background of cirrhosis can be challenging. In this study, the biomarkers to distinguish HCC from cirrhotic nodules were studied.
Design: Frozen samples from 40 HBV-associated post-operative HCC patients and the clinicopathological data were collected. We used two-dimensional gel electrophoresis(2-DE) coupled with tandem mass spectrometry (MS) to study the proteins differentially expressed in HCC and the adjacent non-HCC cirrhotic tissue. Furthermore, the correlations between potential biomarkers and clinicopathological data of HCC were evaluated using bivariate correlation analysis.
Results: Heterogeneous nuclear ribonucleoprotein K (hnRNP K) was markedly upregulated in HCC compared to the non-HCC cirrhotic tissue. The overexpression of hnRNP K in HCC tissues was further confirmed by Western blot and immunohistochemistry. Receiver operating characteristic (ROC) curve analysis revealed that hnRNP K in combination with serum AFP was a sensitive (93.3%) and specific (96.0%) marker to detect HCC in HBV infected cirrhotic liver tissues.
Conclusions: The study revealed overexpression of hnRNP K in HCC which could be a potential diagnostic marker and a candidate target for therapy.
Category: Special Category - Pan-genomic/Pan-proteomic approaches to Cancer
Tuesday, March 20, 2012 9:30 AM
Poster Session III # 273, Tuesday Morning