Association of Chlamydophila Psittaci in Cases of Ocular Adnexal MALT Lymphoma
Gizem Tumer, Helen Fernandes, Anjali Seth, Neena Mirani. UMDNJ-University Hospital, Newark, NJ
Background: Extranodal marginal zone B-cell lymphoma of mucosa associated lymphoid tissue type (MALT lymphoma) is the most common type of malignant lymphoma of the ocular adenexa. Recently several studies have indicated that Chlamydophila psittaci (C.psittaci) has been associated to ocular adenexal lymphomas with variable geographic distribution. This observation points toward the opportunity to investigate the prevalance of C.Psittaci infection in ocular adenexal MALT lymphoma.
Design: Nine patients with ten cases of biopsy proven ocular adenexal MALT lymphoma from over the past 10 years (from January 1st 2001 to August 31st 2011) were selected from the archived file. Paraffin embedded, formalin fixed specimens were studied for C.Psittaci by immunohistochemistry and molecular analyses.
Immunohistochemistry was performed using anti-genus-specific lipopolysaccharide polyclonal antibody (BDI168, Santa Cruz Biotechnology). The presence of C.psittaci was determined by amplification of two separate targets of the C.psittaci genome. Nested PCR for the 16S-23S spacer region was performed using touchdown PCR followed by realtime amplification with visualization of melt curves. The second target used for identification was the omp 1 gene of C. psittaci. A real time PCR with primers and FRET probes were used for amplification.
Results: In our study four of ten biopsy cases show immunoreactivity to anti-genus-specific lipopolysaccharide polyclonal antibody indicating that Chlamydial species are present in the lesional tissue. Furthermore, none of the specimens tested showed amplification for C.psittaci by molecular studies. The positive control used was successfully amplified and confirmed by sequencing for both targets.
Conclusions: In ten-year retrospective study of ocular MALT lymphomas in our institution, we could not identify C.psittaci by molecular studies. Our results correlate well with the current literature that C.psittaci vary widely between geographic regions and even within the same country. The prevalence range varies from 11-50%. Further collaborative large international study is still necessary to clarify the role of C.Psittaci in the pathogenesis of the disease.
Wednesday, March 21, 2012 1:00 PM
Poster Session VI # 312, Wednesday Afternoon