Expression of Calretinin and CK5/6 in Cholangiocarcinoma
Lanjing Zhang, Renee Frank, Emma E Furth, Amy F Ziober, Viginia A LiVolsi, Paul J Zhang. University Medical Center at Princeton, Princeton, NJ; University of Pennsylvania Medical Center, Philadelphia, Philadelphia, PA
Background: One of the mesothelial lineage markers, mesothelin, has been found expressed in, and a potential treatment target of, cholangioacarcinoma (CC). It raises the possibility that CC may be derived from cells sharing or switching to mesothelial phenotype. However, the expression of other mesothelial markers in CC is largely unknown.
Design: Twenty three well documented CC cases were retrieved from the archive of our institution. Immunohistochemical study of Calretinin (DC8), WT1 (6F-H2), D2-40, CK5/6 (D5/16 B4) and CK19 (b170) was done on formalin fixed paraffin embedded sections from each case using standard immunohistochemical protocols. We compared the protein expression levels between CC and normal bile duct (NBD) on the same block.
Results: All of the CC and BD are positive for CK19 (23/23), and all or mostly negative for WT1 (0/23) and D2-40 (1/23). Interestingly, calretinin immunoreactivity was detected in 52.2% (12/23) of CC, but not in NBD (0/23). CK5/6 was also detectable in 73.9% (17/23) of CC as well as all NBD (23/23) (Table).
|Positive (total)||12 (52.2%)||0||17 (73.9%)||23 (100%)|
|1+||4 (17.4%)||0||6 (26.1%)||2 (8.7%)|
|2+||4 (17.4%)||0||4 (17.4%)||6 (26.1%)|
|3+||4 (17.4%)||0||7 (30.4%)||15 (65.2%)|
|Total||23 (100%)||23 (100%)||23 (100%)||23 (100%)|