Whole Slide Imaging System Resolution Evaluation Slide Set Development: Toward Standardization
Yukako Yagi, Pinky A Bautista. Massachusetts General Hospital, Boston, MA
Background: System resolution of Whole Slide Imaging (WSI) system is very important toward standardization. While the most common reason for the variations of color and image quality is the variance in the protocols and practices in the histology lab, the image displayed can also be affected by variation in capture parameters, image processing and display factors in the digital systems themselves. It is difficult to identify which exactly causes the problem. We have developed the methodology to evaluate the balance as a system and identify the cause of the problem of image quality, color and system resolution. In addition to the two calibration slides we have developed for color and image quality, we developed another slide to evaluate optics of the WSI.
Design: In addition to the two calibration slides we have, we developed a slide to evaluate the optics of whole slide imaging system. Using the two calibration slides we can evaluate and standardize whole slide imaging but we can not identify exact cause of the quality issue in the system. These three slides were scanned by several different manufactures' scanner. From the corresponding whole slide images of the two calibration slides we previously developed we evaluated the color characteristics of the scanner and the image quality of the whole slide images each scanner produced. On the other hand, from the scanned image of the new slide, we evaluated the balance between optics, electronics and software and determined the cause of image quality degradation and color variations.
Results: The usefulness of the color and image quality evaluation slides was demonstrated. Furthermore, the new slide for Optics evaluation showed interesting results. Some system showed very good results in the Image Quality Evaluation and Color however, the result for the new slide was not good.
After the investigation of the system's component, we found that the system has relatively lower optics quality than image acquisition device If optics quality is improved, it is expected that the system resolution would also improve.
Conclusions: The importance of the additional slide to evaluate the optics of WSI from many aspects was demonstrated. The new slide could help improve system resolution of Whole Slide Imaging System toward Standardization.
Tuesday, March 20, 2012 1:00 PM
Poster Session IV # 222, Tuesday Afternoon