Histopathologic Correlation with Spectral-Domain Optical Coherence Tomography (SD-OCT), a Novel Digital Imaging Technique
Matthew Smith, Larry Kagemann, Joel S Schuman, Jeffrey L Fine. University of Pittsburgh, Pittsburgh, PA
Background: Spectral-Domain optical coherence tomography (SD-OCT) is a novel 3D imaging technique that can produce images that resemble low-to-medium magnification photomicrographs directly from tissue, without glass microscope slides. In addition to potentially decreasing turn-around time for pathologic diagnosis, such 3D imaging is an opportunity to augment current histopathology knowledge. This project is an effort to characterize the 3D histopathology of vulvar Paget's disease using spectral-domain OCT.
Design: SD-OCT images were acquired from formalin-fixed, paraffin-embedded tissue blocks of vulvar Paget's disease (Bioptigen, Research Triangle, North Carolina, USA). 2 mm deep volumes of tissue were sampled with 500 x 500 x 1024 voxel resolution over small areas of the block (transverse resolution 20 microns, axial resolution 2 microns). OCT images underwent post-processing then were correlated with routine H&E stained slides. Virtual slices and 3D reconstructions were created.
Results: Low-magnification features were recognizable including nests of Paget's cells. Overlying hyperkeratosis, epidermal proliferation and dermis are all distinctly visible in the OCT images. Initial OCT images lacked resolution and contrast relative to traditional microscopy, but the image content suggests that additional features of interest are present and may be revealed with improved imaging technique and post-processing experience.
Conclusions: Rapid or near-instant diagnosis (either in vivo or in vitro) is a tremendous opportunity for pathologists. It is also a challenge, in that existing histopathology expertise must be expanded to include 3D information. As with previous SD-OCT images in pathology, resolution and contrast are less than with standard microscopy—this is being addressed with improvements to imaging technique and with new OCT systems that are optimized for pathology specimens. Finally, existing images will be useful in creating computer-assisted diagnosis systems.
Tuesday, March 20, 2012 1:00 PM
Poster Session IV # 215, Tuesday Afternoon