DC-SCRIPT Expression in Diffuse Large B-Cell Lymphoma with Clinical Correlation
Li Li, Katsiaryna Laziuk, Christine Sheehan, Tipu Nazeer. Albany Medical College, Albany, NY
Background: DC-SCRIPT (Dendritic cell-specific transcript), also known as Zinc finger protein 366, is a protein encoded by ZNF366 gene. It is a nuclear receptor co-regulator that activates and represses the transcriptional activity of certain nuclear receptors. It is found to be expressed in all dendritic cells. It has been shown that a higher level of DC-SCRIPT is an independent prognostic factor for primary breast cancer, associated with favorable outcomes. DC-SCRIPT has not been widely studied in clinical specimens of hematologic malignancies.
Design: Formalin-fixed, paraffin embedded sections from 82 Diffuse Large B-cell lymphomas (DLBCL), were immunostained by automated methods (Ventana Medical Systems, Inc, Tucson, AZ) using goat anti-human DC-SCRIPT/ZNF366 antibody (R&D Systems, Minneapolis, MN). Cytoplasmic immunoreactivity was semiquantitatively assessed in all cases. Scoring was based on staining intensity (weak, moderate, intense) and percentage of positive cells (focal <= 10%, regional 11-50%, diffuse >50%). Results were correlated with clinicopathologic variables.
Results: Immunoreactivity was predominantly cytoplasmic for the DC-SCRIPT protein. DC-SCRIPT over-expression was noted in 55/82 (67%) of cases and correlated with nodal disease [30/35 (86%) nodal versus 25/47 (53%) extranodal, p=0.002] and disease remission [12/17 (71%) in remission versus 1/5 (20%) not in remission, p=0.043, remission status available in 22 cases]. In the HIV+ subgroup, DC-SCRIPT over-expression reached near significant correlation with advanced stage [6/6 (100%) advanced stage versus 5/9 (56%) early stage, p=0.057] and nodal disease [5/5 (100%) nodal versus 8/14 (57%) extranodal, p=0.077].
Conclusions: Cytoplasmic DC-SCRIPT is expressed in the majority of DLBCLs and is significantly associated with nodal disease and remission status. In the HIV positive subgroup, DC-SCRIPT immunoreactivity is associated with advanced stage and nodal disease. Further studies in DLBCL and in particular HIV-associated DLBCL are warranted.
Tuesday, March 20, 2012 1:00 PM
Poster Session IV # 197, Tuesday Afternoon