Limited Flow Cytometry Panels in the Evaluation of Bone Marrow Samples
Douglas G Hoffmann, Burton H Kim. Wellstar Health System, Marietta, GA
Background: A comparison of limited and unlimited flow cytometry panels of bone marrow aspirate samples has not been reported. In this study, we compare a limited flow cytometry strategy (LS) to a broad multi-marker panel (MMS) on bone marrow samples to determine whether the use of limited panels impacts disease detection in these samples.
Design: We reviewed bone marrow morphology and flow cytometry of 1246 consecutive bone marrow samples received from August 2009 to December, 2010. One facility received 488 samples where an MMS strategy was employed. At a second hospital, 758 bone marrow samples were examined using an LS strategy.
|Limited Panel Strategy||Multi-marker Strategy|
|Number of cases||758||488|
|Average number of flow markers per case||11.6||22|
|% positive cases by flow||26.7||22|
|% positive cases by flow or histology||37.9||28.7|
|number of IHC stains per case||0.15||0.23|