Identification of Novel Antigens To Identify the Neoplastic Cells of Nodular Lymphocyte Predominant Hodgkin Lymphoma Using a Lyoplate
Jonathan R Fromm, David Wu, Brent L Wood. University of Washington, Seattle, WA
Background: Nodular lymphocyte predominant Hodgkin's lymphoma (NLPHL) is usually confirmed by immunohistochemistry, but challenging cases exist in which this neoplasm can be difficult to differentiate from classical Hodgkin lymphoma (CHL) and B cell non-Hodgkin lymphomas.
Design: We sought to identify potentially useful novel antigens for the identification of L&H cells (neoplastic cells of NLPHL) using a Lyoplate™ (unconjugated antibodies to 242 cell surface antigens in 96-well plates). Antigen expression of a NLPHL cell line (DEV) was compared to a CHL cell line (L1236) and a Burkitt lymphoma cell line (Raji). Aliquots of the 3 cell lines were labeled with different fluorochromes (“barcoded”), processed with the antibody reagents, and evaluated by flow cytometry. Mean fluorescence intensity (MFI) of antigen expression by the different cell lines were measured and the MFI ratios calculated.
Results: Expression of antigens on DEV relative to the Raji and L1236 cells is shown in Table 1.
|↑ relative to Raji||↓ relative to Raji||↑ relative to L1236||↓ relative to L1236||↑ relative to Raji & L1236||↓ relative to Raji & L1236|
|CD30 CD39 CD44 CD59 CD62L CD79b CD87 CD106 CD108 CD146 CD171 CD273 CD275||CD10 CD21 CD27 CD32 CD38 CD58 CD72 CD85 CD49F CD97||CD19 CD20 CD22 CD37 CD39 CD45RB CD45RA CD50 CD53 CD62L CD75 CD79b CD71 CD81 CD87 CD102 CD146 CD171 CD268 CD275||CD15 CD26 CD32 CD31 CD58 CD97 CD227 CD274||CD39 CD87 CD275||CD32 CD97|
|HLA-ABC B2M HLA-DR HLA DQ HLA-DP/DR/DQ||B2M HLA-DQ HLA-ABC DIS GD2 HLA-A2 HLA-DR/DQ/DP HLA-DR SSEA-1|