Automated Image Analysis and Scoring of Tartrate Resistance Acid Phosphatase Preparations
Bryan Dangott, Mohamed Salama. University of Utah, Salt Lake City, UT
Background: Whole slide image (WSI) analysis has great potential for standardizing diagnostic interpretation, streamlining workflow, and improving patient care. Traditionally image analysis is performed on a small area of the slide that is pre-selected by a trained user. This pre-analytical step can disrupt workflow and thus hinder the use of these techniques. Our solution was to develop algorithms to automatically find the relevant area of interest on a native, unannotated whole slide image. The analysis was performed on WSI of peripheral blood smears prepared with tartrate resistant acid phosphatase (TRAP) staining. Lymphocytes were automatically scored and presented to the pathologist in the form of a histogram, high magnification thumbnail images, or a fully annotated digital slide.
Design: Randomly selected peripheral blood slides of TRAP preparations were scanned at 40x using an Aperio CS scanner. Definiens Developer XD was then used to analyze the WSI in their native state without pre-annotation or pre-selection of the relevant areas of interest. The algorithm automatically detected the region of interest (ROI) and performed scoring of the lymphocytes on a scale of 0 through 5 for their level of staining.
Results: A set of 40 slides was created for the experiment. Slides were scored by the alogrithm with automatic detection of the ROI. The summary histogram provided a snapshot of the results. The extracted thumbnails provided extremely rapid review and visual confirmation of the results at full magnification in a consolidated format. Automatic WSI annotation allowed the results to be viewed in the context of the original slide.
Conclusions: Using the automation techniques described above we were able to realize several advantages over current methods. First the slides can be completely and automatically pre-scored prior to sending the case to the pathologist. In addition, the case can be reviewed rapidly and even remotely using the extracted thumbnail images. Alternatively, the original whole slide image with annotated, scored lymphocytes can be viewed. This technique allows thousands of cells to be evaluated in less time than traditional methods.
Wednesday, March 21, 2012 9:30 AM
Poster Session V # 212, Wednesday Morning