CTNNB1 Mutation, ß-Catenin and E-Cadherin Expression and the Relationship with Clinical and Histopathological Prognostic Factors in Endometrial Adenocarcinomas and Hyperplasias
Naciye Ozeren, Derya Gumurdulu, Baris Guzel, Handan Zeren, Melek Ergin, Mehmet Ali Vardar, Aytekin Altintas. Cukurova University, Faculty of Medicine, Adana, Turkey
Background: Endometrial cancers are the most common cancers of the female genital tract that more than 80% of it consist of endometrioid type adenocarcinomas. In endometrial carcinomas, the underlying factors and their correlation with several histologic and clinical parameters are guiding the patients follow up and new treatment methods. In our study, CTNNB1 mutation, β-catenin and E-cadherin expressions are examined and their relationship with the prognostic parameters and survival are studied.
Design: To the study, ninety three endometrioid adenocarcinoma, and fifteen atypical endometrial hyperplasia cases were included and those cases were diagnosed at Cukurova University, Medical Faculty at the Pathology Department between the years 2002-2007. We have investigated the expression of β-catenin, E-cadherin by immunohistochemistry and CTNNB1 mutation by PCR in all cases.
Results: In 93 endometrioid adenocarcinoma cases, membranous and cytoplasmic β-catenin expression was found in 74 cases, nuclear β-catenin expression was found in 14 cases, negative β-catenin expression was found in 5 cases. All of the 15 atypical endometrial hyperplasia cases displayed cytoplasmic and membranous β-catenin expression. In univariete analysis, β-catenin was found to have prognostic significance with survival. In multivariete analysis, negative β-catenin expression was found as an independent prognostic factor. CTNNN1 mutation was found in 34 (36.6%) of 93 endometrioid adenocarcinoma, and it was found in 3 (20%) cases out of 15 atypical endometrial hyperplasia cases. There was a significant correlation between CTNNB1 mutation and histologic grade. Decreased mutations were found in high grade tumors. There were statistical significance between E-cadherin expression and tumor grade, lymphovascular invasion, and metastasis of lymph node.
Conclusions: In this study, it has been shown that CTNNB1 mutation take a role early steps of carcinogenesis in endometrioid adenocarcinoma and negatif β-catenin expression was found to be an independent prognostic factor for survival.
Category: Gynecologic & Obstetrics
Wednesday, March 21, 2012 9:30 AM
Poster Session V # 143, Wednesday Morning